Amino Acid Sequence: MNFSGKYQLQ SQENFEPFMK AIGLPEDLIQ KGKDIKGVSE IVHEGKKIKL TITYGPKVVR NEFTLGEECE LETMTGEKVK AVVKLEGDNK MVTTFKGIKS VTELNGDTIT NTMTLGDIVY KRVSKRI

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of FABP1/L-FABP as determined by LAL method.

Predicted Molecular Mass

14.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

liver FABP Protein is fully biologically active when compared to standard. The binding affinity of rMuFABP1 for the synthetic ligand cis-parinaric acid has been measured by fluorescence titration. Half maximal fluorescence of 2.5 uM rMuFABP1 is achieved with approximately 5 uM cis-paranaric acid.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Mouse FABP1/L-FABP Protein

SDS-PAGE: Recombinant Mouse FABP1/L-FABP Protein [NBP2-35180]

SDS-Page: Mouse FABP1/L-FABP Protein [NBP2-35180]

Formulation, Preparation, and Storage

NBP2-35180

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4, 2 % trehalose.
Preservative	Trehalose
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FABP1/L-FABP

The fatty-acid-binding proteins (FABPs) are a family of carrier proteins for fatty acids and other lipophilic substances such as eicosanoids and retinoids. These proteins are thought to facilitate the transfer of fatty acids between extra- and intracellular membranes. FABP (FABP1) encoded by the FABP1 gene, also known as liver-type fatty acid-binding protein (L-FABP), is a member of FABP family and it is a small, highly conserved, cytoplasmic proteins. In addition, FABP (FABP1) binds free fatty acids and their coenzyme A derivatives, bilirubin, and some other small molecules in the cytoplasm. Furthermore, it may be involved in intracellular lipid transport. Through amino acid sequence comparison, murine FABP (FABP1) shares 84 % and 94 % a.a. sequence identity with human and rat FABP1.

Long Name

Fatty Acid-Binding Protein 1

Alternate Names

L-FABP, LFABP, FABP1

Gene Symbol

FABP1

UniProt

P12710 (Human)

Additional FABP1/L-FABP Products

Product Documents for Recombinant Mouse FABP1/L-FABP Protein

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Certificate of Analysis

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Product Specific Notices for Recombinant Mouse FABP1/L-FABP Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Related Research Areas

Early Endodermal Lineage Markers

Obesity

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Recombinant Mouse FABP1/L-FABP Protein

Novus Biologicals | Catalog # NBP2-35180

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