Human Factor IX ELISA Kit (Colorimetric)

Key Product Details

Product Specifications

Precision

Intra-Assay Precision (Precision within an assay) %CV 3.50 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV 8.90 (example only; lot dependent)

Recovery for Human Factor IX ELISA Kit (Colorimetric)

Scientific Data Images for Human Factor IX ELISA Kit (Colorimetric)

Kit Contents for Human Factor IX ELISA Kit (Colorimetric)

• Biotinylated Human Factor IX Antibody (50X) - 120 uL
• Chromogen Substrate (1X) - 80 uL
• EIA Diluent (10X) - 30 mL
• Human Factor IX microplate - 12 strips of 8 wells
• Human Factor IX Standard - 80 ng
• Negative Control - 1 vial
• Plate sealing tapes - 3 EA
• Positive Control - 1 vial
• SP Conjugate (100X) - 80 uL
• Stop Solution (1X) - 12 mL
• Wash Buffer (20X) - 2 x 30 mL

Preparation and Storage

Background: Coagulation Factor IX

Factor IX encodes vitamin K-dependent coagulation factor IX that circulates in the blood as an inactive zymogen. This factor is converted to an active form by factor XIa, which excises the activation peptide and thus generates a heavy chain and a light chain held together by one or more disulfide bonds. The role of this activated factor IX in the blood coagulation cascade is to activate factor X to its active form through interactions with Ca+2 ions, membrane phospholipids, and factor VIII. Alterations of this gene, including point mutations, insertions and deletions, cause factor IX deficiency, which is a recessive X-linked disorder, also called hemophilia B or Christmas disease.

Additional Coagulation Factor IX Products

• All Products for Coagulation Factor IX
• Coagulation Factor IX ELISA Kits
• Coagulation Factor IX Lysates
• Coagulation Factor IX Primary Antibodies
• Coagulation Factor IX Proteins and Enzymes

Product Documents for Human Factor IX ELISA Kit (Colorimetric)