Factor V Antibody (HL2421) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-25459

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL2421 expressed in HEK293

Format

Azide and BSA Free
View all Coagulation Factor V Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment of human Factor V.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Factor V Antibody (HL2421) - Azide and BSA Free

Factor V Antibody (HL2421) - Azide and BSA Free

Western Blot: Factor V Antibody (HL2421) - Azide and BSA Free [NBP3-25459] -

Western Blot: Factor V Antibody (HL2421) - Azide and BSA Free [NBP3-25459] - Rat plasma (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Factor V antibody [HL2421] (NBP3-25459) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Factor V Antibody (HL2421) - Azide and BSA Free

Western Blot: Factor V Antibody (HL2421) - Azide and BSA Free [NBP3-25459] -

Western Blot: Factor V Antibody (HL2421) - Azide and BSA Free [NBP3-25459] - Human plasma (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Factor V antibody [HL2421] (NBP3-25459) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Factor V Antibody (HL2421) - Azide and BSA Free

Immunohistochemistry-Paraffin: Factor V Antibody (HL2421) [NBP3-25459] -

Factor V antibody [HL2421] detects secreted Factor V protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat spleen.
Factor V stained by Factor V antibody [HL2421] (NBP3-25459) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications for Factor V Antibody (HL2421) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Coagulation Factor V

The F5 gene encodes a 2,224 amino acid long, 251 kDA coagulation factor V protein that is critical in the regulation of homeostasis. Additionally, F5 functions as a cofactor for the prothrombinase activity of factor Xa which results in the initiation of prothrombin to thrombin. F5 participates in blood coagulation signaling pathways, the blood clotting cascade, platelet degranulation as well as activation, signaling, and aggregation, and in responses to elevated platelet cytosolic Ca2+. It interacts with genes PROC, PROS1, F2, MMRN1, and CALR. Defects in F5 cause factor 5 deficiency (owren parahemophilia), thrombophilia due to activated protein C resistance, susceptibility to Budd-Chiari syndrome, susceptibility to ischemic strokes, and susceptibility to pregnancy loss, recurrent, type 1. F5 is also linked to deep vein thrombosis, antithrombin III deficiency, retinal vein occlusion, legg-calve-perthes disease, liver disease, hypertension, pulmonary embolism, lupus, and patent foramen ovale.

Long Name

Coagulation Factor V

Alternate Names

Cf-5, Cf5, Coagulation Factor 5, F5, Factor V, FVL, PCCF, Proaccelerin, Labile Factor, RPRGL1, THPH2

Gene Symbol

F5

Additional Coagulation Factor V Products

Product Documents for Factor V Antibody (HL2421) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Factor V Antibody (HL2421) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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