FANCD2 Antibody (FI-17) - BSA Free

Novus Biologicals | Catalog # NB100-316

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Hamster (Negative), Mouse (Negative)

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # FI-17

Format

BSA Free
View all FANCD2 Primary Antibodies »

Product Specifications

Immunogen

This FANCD2 Antibody (FI-17) was developed against human FANCD2 fusion protein [Swiss-Prot: Q9BXW9]

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

164.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for FANCD2 Antibody (FI-17) - BSA Free

Simple Western: FANCD2 Antibody (FI-17) [NB100-316]

Simple Western: FANCD2 Antibody (FI-17) [NB100-316]

Simple Western: FANCD2 Antibody (FI-17) [NB100-316] - Image shows a specific band for FANCD2 in 1 mg/mL of HeLa cell lysate using FANCD2 Antibody (FI-17). This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Western Blot: FANCD2 Antibody (FI-17) [NB100-316]

Western Blot: FANCD2 Antibody (FI-17) [NB100-316]

Western Blot: FANCD2 Antibody (FI-17) [NB100-316] - MCF7 cell line (lane 1, molecular weight: 164.1 KDa) and HeLa whole cell extract (lane 2) using FANCD2 Antibody (FI-17).
Western Blot: FANCD2 Antibody (FI-17) [NB100-316]

Western Blot: FANCD2 Antibody (FI-17) [NB100-316]

Western Blot: FANCD2 Antibody (FI-17) [NB100-316] - Expression of FANCD2 (Molecular weight: 164.1 KDa) in different cell lines. Proliferating cells were treated with 10-Gy IR. 50 ug protein was loaded each lane. FANCD2 Antibody (FI-17) at 1:1000.

Applications for FANCD2 Antibody (FI-17) - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1:10-1:500

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Simple Western

1:50

Western Blot

1:1000 - 1:2000
Application Notes
In Western blot, this antibody recognizes a band at ~155 and ~162 kDa (post-translationally modified form). The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in HeLa lysate 1.0 mg/mL, separated by Size, antibody dilution of 1:50, apparent MW was 150 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: FANCD2

Fanconi Anemia (FA) is an autosomal-recessive cancer-prone disorder characterized by congenital defects, progressive bone marrow failure, increased chromosomal breakage, defective DNA repair and cellular hypersensitivity to mitomycin C. Fanconi Anemia Complementation Group D (FANCD) is comprised of two separate proteins, FANCD1/BRCA2 and FANCD2. LYRIC/MTDH, an RNA binding protein, regulates expression of FANCD2 and FANCD1 (1). FANCD2 is mapped to chromosome 3p25.3, has a theoretical molecular weight of 166 kDa and is localized in the nucleus. FANCD2 is involved in the regulation of DNA-binding transcription factor activity and DNA stability through accurate and efficient pairing of homologs during meiosis to promote repair of double-strand DNA breaks (2). Ubiquitination is required for FANCD2 to bind to chromatin. In response to DNA damage, FANCD2 is monoubiquinated to result in colocalization with other proteins (BRCA1 and BRCA2) involved in homology-directed DNA repair in the nucleus and is deubiquitinated upon DNA repair completion.

References

1. Bi, J., Areecheewakul, S., Li, Y., Yang, S., Zhang, Y., Ebeid, K.,... Meng, X. (2019). MTDH/AEG-1 downregulation using pristimerin-loaded nanoparticles inhibits Fanconi anemia proteins and increases sensitivity to platinum-based chemotherapy. Gynecol Oncol, 155(2), 349-358. doi:10.1016/j.ygyno.2019.08.014

2. Balcerek, J., Jiang, J., Li, Y., Jiang, Q., Holdreith, N., Singh, B.,... Tong, W. (2018). Lnk/Sh2b3 deficiency restores hematopoietic stem cell function and genome integrity in Fancd2 deficient Fanconi anemia. Nat Commun, 9(1), 3915. doi:10.1038/s41467-018-06380-1

Long Name

Fanconi Anemia, Complementation Group D2

Alternate Names

FA4, FACD, FAD, FAD2, FANCD

Entrez Gene IDs

2177 (Human); 211651 (Mouse)

Gene Symbol

FANCD2

UniProt

Q9BXW9

Additional FANCD2 Products

Product Documents for FANCD2 Antibody (FI-17) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for FANCD2 Antibody (FI-17) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for FANCD2 Antibody (FI-17) - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for FANCD2 Antibody (FI-17) - BSA Free

Showing  1 - 2 of 2 FAQs Showing All

  • Q: Can you provide the aa sequence used to generate FANCD2 Antibody (FI-17)? Is it the full length protein? In addition, do you know if this antibody has been shown to recognize isoform 1 and 2 of ID: Q9BXW9?

    A: This antibody was generated against the full-length protein. Based on the testing data in our second, cleaner image, this antibody appears to recognize two very close bands in lane 2. These are most likely isoforms 1 and 2.

  • Q: Do you know if this antibody has been shown to recognize isoform 1 and 2 of ID: Q9BXW9?

    A: Based on the testing data in our second, cleaner image, this antibody appears to recognize two very close bands in lane 2. These are most likely isoforms 1 and 2.

  • Q: Can you provide the aa sequence used to generate FANCD2 Antibody (FI-17)? Is it the full length protein? In addition, do you know if this antibody has been shown to recognize isoform 1 and 2 of ID: Q9BXW9?

    A: This antibody was generated against the full-length protein. Based on the testing data in our second, cleaner image, this antibody appears to recognize two very close bands in lane 2. These are most likely isoforms 1 and 2.

  • Q: Do you know if this antibody has been shown to recognize isoform 1 and 2 of ID: Q9BXW9?

    A: Based on the testing data in our second, cleaner image, this antibody appears to recognize two very close bands in lane 2. These are most likely isoforms 1 and 2.

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