FARSB Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-35538

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all FARSB Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 240-589 of human FARSB (NP_005678.3).

Sequence:
DHSRITVNTRNIFIECTGTDFTKAKIVLDIIVTMFSEYCENQFTVEAAEVVFPNGKSHTFPELAYRKEMVRADLINKKVGIRETPENLAKLLTRMYLKSEVIGDGNQIEIEIPPTRADIIHACDIVEDAAIAYGYNNIQMTLPKTYTIANQFPLNKLTELLRHDMAAAGFTEALTFALCSQEDIADKLGVDISATKAVHISNPKTAEFQVARTTLLPGLLKTIAANRKMPLPLKLFEISDIVIKDSNTDVGAKNYRHLCAVYYNKNPGFEIIHGLLDRIMQLLDVPPGEDKGGYVIKASEGPAFFPGRCAEIFARGQSVGKLGVLHPDVITKFELTMPCSSLEINVGPFL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

66 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for FARSB Antibody - BSA Free

FARSB Antibody

Immunocytochemistry/ Immunofluorescence: FARSB Antibody [NBP3-35538] -

Immunocytochemistry/ Immunofluorescence: FARSB Antibody [NBP3-35538] - Immunofluorescence analysis of C6 cells using FARSB Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
FARSB Antibody

Western Blot: FARSB Antibody [NBP3-35538] -

Western Blot: FARSB Antibody [NBP3-35538] - Western blot analysis of various lysates using FARSB Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

Applications for FARSB Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:200 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: FARSB

FARSB encodes a highly conserved enzyme that belongs to the aminoacyl-tRNA synthetase class IIc subfamily. This enzyme comprises the regulatory beta subunits that form a tetramer with two catalytic alpha subunits. In the presence of ATP, this tetramer is responsible for attaching L-phenylalanine to the terminal adenosine of the appropriate tRNA. A pseudogene located on chromosome 10 has been identified.

Alternate Names

EC 6.1.1, EC 6.1.1.20, FARSLBphenylalanine-tRNA ligase beta chain, FRSBphenylalanine tRNA ligase 1, beta, cytoplasmic, PheHB, Phenylalanine--tRNA ligase beta chain, phenylalanine-tRNA synthetase-like, beta subunit, phenylalanyl-tRNA synthetase beta chain, phenylalanyl-tRNA synthetase beta-subunit, phenylalanyl-tRNA synthetase, beta subunit, phenylalanyl-tRNA synthetase-like, beta subunit, PheRS

Gene Symbol

FARSB

Additional FARSB Products

Product Documents for FARSB Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for FARSB Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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