FASTK Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-31323

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Canine (95%), Porcine (90%), Primate (99%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human FASTK. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Chimpanzee (99%), Bovine (88%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

61 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for FASTK Antibody - BSA Free

Western Blot: FASTK Antibody [NBP1-31323]

Western Blot: FASTK Antibody [NBP1-31323]

Western Blot: FASTK Antibody [NBP1-31323] - A. 30 ug PC-12 whole cell lysate/extract 7.5 % SDS-PAGEFAST antibody dilution: 1:1000
Immunocytochemistry/ Immunofluorescence: FASTK Antibody [NBP1-31323]

Immunocytochemistry/ Immunofluorescence: FASTK Antibody [NBP1-31323]

Immunocytochemistry/Immunofluorescence: FASTK Antibody [NBP1-31323] - Analysis of methanol-fixed HeLa, using FAST antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: FASTK Antibody [NBP1-31323]

Immunohistochemistry-Paraffin: FASTK Antibody [NBP1-31323]

Immunohistochemistry-Paraffin: FASTK Antibody [NBP1-31323] - Paraffin-embedded SCC15 xenograft, using antibody at 1:100 dilution.
Western Blot: FASTK Antibody [NBP1-31323]

Western Blot: FASTK Antibody [NBP1-31323]

Western Blot: FASTK Antibody [NBP1-31323] - A:NIH-3T3 7.5% SDS PAGE diluted at 1:1000
Western Blot: FASTK Antibody [NBP1-31323]

Western Blot: FASTK Antibody [NBP1-31323]

Western Blot: FASTK Antibody [NBP1-31323] - A. 30 ug 293T whole cell lysate/extractB. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract E. 30 ug A375 whole cell lysate/extract7.5 % SDS-PAGEFAST antibody dilution: 1:1000
FASTK Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: FASTK Antibody [NBP1-31323] -

FASTK antibody detects FASTK protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: FASTK protein stained by FASTK antibody (NBP1-31323) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 um.

Applications for FASTK Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: FASTK

The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase was shown to be activated rapidly during Fas-mediated apoptosis in Jurkat cells. In response to Fas receptor ligation, it phosphorylates TIA1, an apoptosis-promoting nuclear RNA-binding protein. The encoded protein is a strong inducer of lymphocyte apoptosis. Two transcript variants encoding different isoforms have been found for this gene. Other variants exist, but their full-length natures have not yet been determined.

Alternate Names

EC 2.7.11.8, Fas-activated serine/threonine kinase, FASTFAST kinase

Gene Symbol

FASTK

Additional FASTK Products

Product Documents for FASTK Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for FASTK Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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