FBXO22 Antibody (OTI1H1)

Novus Biologicals | Catalog # NBP3-44826

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1H1
View all FBXO22 Primary Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-220 of human FBXO22 (NP_671717) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for FBXO22 Antibody (OTI1H1)

FBXO22 Antibody (OTI1H1) Simple Western;FBXO22 Antibody (OTI1H1)[NBP3-44826]-

Simple Western;FBXO22 Antibody (OTI1H1)[NBP3-44826]-

Simple Western™ analysis of endogenous protein FBXO22 from HELA lysates (0.1 mg/mL) using FBXO22 Mouse Monoclonal Antibody #TA809766. The virtual lane view (left) shows the target (as indied) at a 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminey molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12–230 kDa Separation Module.
FBXO22 Antibody (OTI1H1) Western Blot;FBXO22 Antibody (OTI1H1)[NBP3-44826]-

Western Blot;FBXO22 Antibody (OTI1H1)[NBP3-44826]-

Western blot analysis of extracts (35ug) from 2 different cell lines and mouse heart tissue lysate by using anti-FBXO22 monoclonal antibody (1:500).
FBXO22 Antibody (OTI1H1) Western Blot;FBXO22 Antibody (OTI1H1)[NBP3-44826]-

Western Blot;FBXO22 Antibody (OTI1H1)[NBP3-44826]-

HEK293T cells were transfected with the pCMV6-ENTRY control ( Left lane) or pCMV6-ENTRY FBXO22 ( Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FBXO22(1:2000). Positive lysates

Applications for FBXO22 Antibody (OTI1H1)

Application
Recommended Usage

Western Blot

1:500-2000

Formulation, Preparation, and Storage

Purification

>95%

Reconstitution

For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized powder ( 1X PBS, pH 7.3,)

Preservative

8% Trehalose

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 degrees C. Avoid freeze/thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FBXO22

Alternate Names

F-box only protein 22, F-box protein 22, F-box protein FBX22p44, FBX22FLJ13986, FIST domain containing 1, FISTC1, MGC31799

Gene Symbol

FBXO22

Additional FBXO22 Products

Product Documents for FBXO22 Antibody (OTI1H1)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for FBXO22 Antibody (OTI1H1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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