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Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot, Simple Western
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI1H1
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Product Specifications
Immunogen
Human recombinant protein fragment corresponding to amino acids 1-220 of human FBXO22 (NP_671717) produced in E.coli.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for FBXO22 Antibody (OTI1H1)
FBXO22 Antibody [NBP3-45022]
Analysis of extracts (35ug) from 2 different cell lines and mouse heart tissue lysate by using anti-FBXO22 monoclonal antibody (1:500).FBXO22 Antibody NBP3-45022]
HEK293T cells were transfected with the pCMV6-ENTRY control Left lane) or pCMV6-ENTRY FBXO22 Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FBXO22 (1:2000).FBXO22 Antibody [NBP3-45022]
Equivalent amounts of cell lysates (30 ug per lane) of wild-type HeLa cells (WT) and FBXO22-Knockdown HeLa cells (KD) were separated by SDS-PAGE and immunoblotted with anti-FBXO22 monoclonal antibody (1:2500). Then the blotted membrane was stripped and reprobed with anti-HSP90AA1 antibody as a loading control.Simple Western:FBXO22 Antibody (OTI1H1)[NBP3-45022]
Simple Western:FBXO22 Antibody (OTI1H1)[NBP3-45022]Western Blot;FBXO22 Antibody (OTI1H1)[NBP3-45022]-
HEK293T cells were transfected with the pCMV6-ENTRY control ( Left lane) or pCMV6-ENTRY FBXO22 ( Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FBXO22 (1:2000). Positive lysatesWestern Blot;FBXO22 Antibody (OTI1H1)[NBP3-45022]-
Western blot analysis of extracts (35ug) from 2 different cell lines and mouse heart tissue lysate by using anti-FBXO22 monoclonal antibody (1:500).Applications for FBXO22 Antibody (OTI1H1)
Application
Recommended Usage
Simple Western
1:20-1:50
Western Blot
1:500-2000
Formulation, Preparation, and Storage
Purification
>95%
Formulation
PBS (pH 7.3) 1% BSA, 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 degrees C. Avoid freeze/thaw cycles.
Background: FBXO22
Alternate Names
F-box only protein 22, F-box protein 22, F-box protein FBX22p44, FBX22FLJ13986, FIST domain containing 1, FISTC1, MGC31799
Gene Symbol
FBXO22
Additional FBXO22 Products
Product Documents for FBXO22 Antibody (OTI1H1)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for FBXO22 Antibody (OTI1H1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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