FBXO22 Antibody (OTI1H1)

Novus Biologicals | Catalog # NBP3-45022

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1H1
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Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-220 of human FBXO22 (NP_671717) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for FBXO22 Antibody (OTI1H1)

FBXO22 Antibody (OTI1H1)

FBXO22 Antibody [NBP3-45022]

Analysis of extracts (35ug) from 2 different cell lines and mouse heart tissue lysate by using anti-FBXO22 monoclonal antibody (1:500).
FBXO22 Antibody (OTI1H1)

FBXO22 Antibody NBP3-45022]

HEK293T cells were transfected with the pCMV6-ENTRY control Left lane) or pCMV6-ENTRY FBXO22 Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FBXO22 (1:2000).
FBXO22 Antibody (OTI1H1)

FBXO22 Antibody [NBP3-45022]

Equivalent amounts of cell lysates (30 ug per lane) of wild-type HeLa cells (WT) and FBXO22-Knockdown HeLa cells (KD) were separated by SDS-PAGE and immunoblotted with anti-FBXO22 monoclonal antibody (1:2500). Then the blotted membrane was stripped and reprobed with anti-HSP90AA1 antibody as a loading control.
FBXO22 Antibody (OTI1H1)

Simple Western:FBXO22 Antibody (OTI1H1)[NBP3-45022]

Simple Western:FBXO22 Antibody (OTI1H1)[NBP3-45022]
FBXO22 Antibody (OTI1H1) Western Blot;FBXO22 Antibody (OTI1H1)[NBP3-45022]-

Western Blot;FBXO22 Antibody (OTI1H1)[NBP3-45022]-

HEK293T cells were transfected with the pCMV6-ENTRY control ( Left lane) or pCMV6-ENTRY FBXO22 ( Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FBXO22 (1:2000). Positive lysates
FBXO22 Antibody (OTI1H1) Western Blot;FBXO22 Antibody (OTI1H1)[NBP3-45022]-

Western Blot;FBXO22 Antibody (OTI1H1)[NBP3-45022]-

Western blot analysis of extracts (35ug) from 2 different cell lines and mouse heart tissue lysate by using anti-FBXO22 monoclonal antibody (1:500).

Applications for FBXO22 Antibody (OTI1H1)

Application
Recommended Usage

Simple Western

1:20-1:50

Western Blot

1:500-2000

Formulation, Preparation, and Storage

Purification

>95%

Formulation

PBS (pH 7.3) 1% BSA, 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 degrees C. Avoid freeze/thaw cycles.

Background: FBXO22

Alternate Names

F-box only protein 22, F-box protein 22, F-box protein FBX22p44, FBX22FLJ13986, FIST domain containing 1, FISTC1, MGC31799

Gene Symbol

FBXO22

Additional FBXO22 Products

Product Documents for FBXO22 Antibody (OTI1H1)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for FBXO22 Antibody (OTI1H1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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