Fc gamma RIIA/CD32a Antibody (DMC494) - Chimeric

Novus Biologicals | Catalog # NBP3-48372

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Human IgG1 Clone # DMC494
View all Fc gamma RIIA/CD32a Primary Antibodies »

Product Specifications

Immunogen

Fc gamma RIIA/CD32a recombinant protein expressed in mammalian cells (Accession# P12318). The exact amino acid sequence is proprietary.

Clonality

Monoclonal

Host

Human

Isotype

IgG1

Description

This product is shipped at ambient temperature. Store at -20C to -80C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80C (Avoid repeated freezing and thawing).

Scientific Data Images for Fc gamma RIIA/CD32a Antibody (DMC494) - Chimeric

Fc gamma RIIA/CD32a Antibody (DMC494) - Chimeric

Flow Cytometry: Fc gamma RIIA/CD32a Antibody (DMC494) - Chimeric [NBP3-48372]

Flow cytometry analysis of antigen binding of anti-human CD32a mAb(NBP3-48372). (A) NBP3-48372 does not bind to CHO-S cells that do not express CD32a. (B) A clear peak shift of NBP3-48372 was seen compared to the control when incubated with CD32a-expressing THP-1 cells, indicating strong binding of NBP3-48372 to CD32a. Antibodies were incubated at 5 ug/mL.
Fc gamma RIIA/CD32a Antibody (DMC494) - Chimeric

Flow Cytometry: Fc gamma RIIA/CD32a Antibody (DMC494) - Chimeric [NBP3-48372]

Flow cytometry analysis with Anti-Fc gamma RIIA/Fc gamma RIIA/CD32a (DMC494) on HEK293 cells transfected with human Fc gamma RIIA/Fc gamma RIIA/CD32a (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram).

Applications for Fc gamma RIIA/CD32a Antibody (DMC494) - Chimeric

Application
Recommended Usage

Flow Cytometry

1:100

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified from cell culture supernatant

Reconstitution

Dissolve the lyophilized antibody in sterile and low endotoxin water. The recommend volume of water is lot specific. Please refer to the instructions printed on the product vial or contact technical support.

Formulation

PBS, pH 7.4, 5-8% Trehalose

Preservative

Trehalose

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C in powder form. Store at -80C once reconstituted.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Fc gamma RIIA/CD32a

The FCGR2A gene encodes a low affinity immunoglobulin gamma Fc region receptor II-a protein that in isoform 1 is 317 amino acids long at 35 kDA and at isoform 2 is 316 amino acids long at nearly 35 kDA. These proteins are found on the surface of many immune system response cells that act as a low affinity receptor through encouraging responses against pathogens and soluble antigens. Additionally, it functions to initiate phagocytosis of opsonized antigens. FCGR2A participates in Fc-GammaR pathway, osteoclast differentiation, signaling events controlled by PTP1B, and NFAT in immune response. It is known to interact with genes SYK, FYN, HCK, LGALS3, and PIK3R1. FCGR2A is linked to lupus, thrombocytopenia, b-cell lymphomas, otitis media, adult-onset still's disease, guillain-barre syndrome, asymptomatic dengue, severe acute respiratory syndrome.

Long Name

Fc gamma Receptor II A

Alternate Names

CD32a, FCGR2A, FcgRIIA, FCRIIA

Gene Symbol

FCGR2A

Additional Fc gamma RIIA/CD32a Products

Product Documents for Fc gamma RIIA/CD32a Antibody (DMC494) - Chimeric

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Fc gamma RIIA/CD32a Antibody (DMC494) - Chimeric

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for Fc gamma RIIA/CD32a Antibody (DMC494) - Chimeric

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  • Q: Does any of your FCGR2A antibodies (19C10, 6D11, 13G9) cross react to FCGR2B?

    A: For these antibodies, we have used the full-length protein FCGR2A (NP_067674) as the immunogen and we have not mapped the epitope that is detected with each Ab yet. I did an alignment of these two proteins and they are highly conserved thus it seems likely that there will be cross-reactivity.

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