Fc gamma RIIA/CD32a Antibody (IV.3) - Chimeric - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-20159

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Block/Neutralize, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Inhibition Activity

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # IV.3

Format

Azide and BSA Free
View all Fc gamma RIIA/CD32a Primary Antibodies »

Product Specifications

Immunogen

The original antibody was generated against the FcR of K562 cells.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images for Fc gamma RIIA/CD32a Antibody (IV.3) - Chimeric - Azide and BSA Free

Fc gamma RIIA/CD32a Antibody (IV.3) - Chimeric - Azide and BSA Free

Flow Cytometry: Fc gamma RIIA/CD32a Antibody (IV.3) - Chimeric - Azide and BSA Free [NBP3-20159] -

Flow Cytometry: Fc gamma RIIA/CD32a Antibody (IV.3) - Chimeric - Azide and BSA Free [NBP3-20159] - Paraformaldehyde fixed U937 cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of IV.3 (NBP3-20159, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
Fc gamma RIIA/CD32a Antibody (IV.3) - Chimeric - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: Fc gamma RIIA/CD32a Antibody (IV.3) - Chimeric - Azide and BSA Free [NBP3-20159] -

Immunocytochemistry/Immunofluorescence: Fc gamma RIIA/CD32a Antibody (IV.3) - Chimeric - Azide and BSA Free [NBP3-20159] - Immunofluorescence analysis of paraformaldehyde fixed U937 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of IV.3 (NBP3-20159) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20159, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.

Applications for Fc gamma RIIA/CD32a Antibody (IV.3) - Chimeric - Azide and BSA Free

Application
Recommended Usage

Block/Neutralize

Optimal dilutions of this antibody should be experimentally determined.

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Inhibition Activity

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: Fc gamma RIIA/CD32a

The FCGR2A gene encodes a low affinity immunoglobulin gamma Fc region receptor II-a protein that in isoform 1 is 317 amino acids long at 35 kDA and at isoform 2 is 316 amino acids long at nearly 35 kDA. These proteins are found on the surface of many immune system response cells that act as a low affinity receptor through encouraging responses against pathogens and soluble antigens. Additionally, it functions to initiate phagocytosis of opsonized antigens. FCGR2A participates in Fc-GammaR pathway, osteoclast differentiation, signaling events controlled by PTP1B, and NFAT in immune response. It is known to interact with genes SYK, FYN, HCK, LGALS3, and PIK3R1. FCGR2A is linked to lupus, thrombocytopenia, b-cell lymphomas, otitis media, adult-onset still's disease, guillain-barre syndrome, asymptomatic dengue, severe acute respiratory syndrome.

Long Name

Fc gamma Receptor II A

Alternate Names

CD32a, FCGR2A, FcgRIIA, FCRIIA

Gene Symbol

FCGR2A

Additional Fc gamma RIIA/CD32a Products

Product Documents for Fc gamma RIIA/CD32a Antibody (IV.3) - Chimeric - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Fc gamma RIIA/CD32a Antibody (IV.3) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs for Fc gamma RIIA/CD32a Antibody (IV.3) - Chimeric - Azide and BSA Free

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  • Q: Does any of your FCGR2A antibodies (19C10, 6D11, 13G9) cross react to FCGR2B?

    A: For these antibodies, we have used the full-length protein FCGR2A (NP_067674) as the immunogen and we have not mapped the epitope that is detected with each Ab yet. I did an alignment of these two proteins and they are highly conserved thus it seems likely that there will be cross-reactivity.

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