fd/M13 bacteriophage Antibody - BSA Free

Novus Biologicals | Catalog # NB100-1633

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Virus

Cited:

Human, Virus

Applications

Validated:

ELISA, Flow Cytometry, Lateral Flow Assay

Cited:

ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all fd/M13 bacteriophage Primary Antibodies »

Product Specifications

Immunogen

Repeated injections with fd bacteriophage.

Reactivity Notes

Cross-reacts with fd and M13 bacteriophages

Specificity

The antibody binds specifically to phage coat proteins of fd phage or M13 phage.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for fd/M13 bacteriophage Antibody - BSA Free

Lateral Flow Assay: fd/M13 bacteriophage Antibody [NB100-1633]

Lateral Flow Assay: fd/M13 bacteriophage Antibody [NB100-1633]

fd-M13-bacteriophage-Antibody-Lateral-Flow-Assay-NB100-1633-img0001.jpg
fd/M13 bacteriophage Antibody

Western Blot: fd/M13 bacteriophage Antibody [NB100-1633] -

Western Blot: fd/M13 bacteriophage Antibody [NB100-1633] - Detection of Norwalk VLPs in lateral-flow assay (LFA).Norwalk VLPs in 100 μL are detected using anti-Norwalk antibodies in the test line (T); gold nanoparticle (top row) & antibody-phage construct followed by HRP/anti-M13 conjugate (bottom row). Control line (C) consists of anti-mouse antibodies for the gold nanoparticle LFA & anti-M13 antibodies for the phage LFA. Nitrocellulose FF80HP was used as test membrane, Fusion 5 as sample pad & CF5 as absorbent pad. All images were equally gamma-corrected (gamma-correction factor = 0.45) to compensate for contrast lost in the overexposed, scanned images & better represent the naked-eye appearance of the raw strips. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25978622), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for fd/M13 bacteriophage Antibody - BSA Free

Application
Recommended Usage

ELISA

1:1000 (indirect)
Application Notes
Use in FLOW reported in scientific literature (PMID: 27704069).

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

0.01 M phosphate buffered saline, pH 7.4

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: fd/M13 bacteriophage

Can be used as a capture antibody when coated directly on microtiter plates, or as a primary detection antibody for specifically captured M13 or fd phage. May be useful in rapidly sorting large phage display libraries (antibody, peptide etc.) with the expressed proteins fused to either the gene III or gene VIII protein of the filamentous phage.May be used as a reagent in "phage ELISA" offering sensitive and specific activity for detection of recombinant phages.

Alternate Names

major coat protein

Gene Symbol

VIII

UniProt

P69541 (Human)

Additional fd/M13 bacteriophage Products

Product Documents for fd/M13 bacteriophage Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for fd/M13 bacteriophage Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for fd/M13 bacteriophage Antibody - BSA Free

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Protocols

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