Feline Fas/TNFRSF6/CD95 Alexa Fluor® 750-conjugated Antibody

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Feline Fas/TNFRSF6/CD95 Alexa Fluor® 750-conjugated Antibody Summary

Species Reactivity
Detects feline Fas/TNFRSF6/CD95 in direct ELISAs and Western blots. In direct ELISAs and Western blots no cross-reactivity with recombinant human Fas, recombinant mouse Fas, or recombinant rat Fas are observed
Monoclonal Mouse IgG1 Clone # 431014
Mouse myeloma cell line NS0-derived recombinant feline Fas/TNFRSF6/CD95
Accession # NP_001009314
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Alexa Fluor 750 (Excitation= 749 nm, Emission= 775 nm)


Recommended Concentration
Flow Cytometry
0.25-1 µg/106 cells
Feline peripheral blood mononuclear cells treated with PMA and Ca2+ ionomycin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Fas/TNFRSF6/CD95

Feline Fas (fibroblast associated; also named CD95 and APO-1) is a 45 kDa type I transmembrane (TM) glycoprotein that is a member of the TNF receptor superfamily (1-3). The family contains about 30 members, and is characterized by the presence of at least one cysteine-rich domain that contains multiple intrachain disulfide bonds. In general, the superfamily is divided into cytoplasmic death domain (DD) containing, and non-DD containing receptors (3). Feline Fas is synthesized as a 314 amino acid (aa) precursor that contains a 24 aa signal sequence, a 148 aa extracellular region, a 16 aa TM segment, and a 126 aa cytoplasmic tail (4). The extracellular region contains four potential N-linked glycosylation sites plus two distinct cysteine-rich domains of approximately 40 aa each; the cytoplasmic tail shows a 45 aa DD. The extracellular region of feline Fas shares 68%, 65%, 53%, and 58% aa sequence identity to porcine, human, mouse, and rat Fas, respectively. There are five alternate splice forms of feline Fas, which vary from 132 aa to 209 aa in length. All utilize exons 1-3 (aa 1-111) and all are missing the transmembrane segment of the full length form (5). Circulating Fas is reported to be both a dimer and trimer at low ng/mL concentrations. The ligand for Fas is FasL, and Fas ligation activates both the MEK cascade and FADD/caspase-8 pathway (7).

  1. Locksley, R.M. et al. (2001) Cell 104:487.
  2. Gaur, U. and B.B. Aggarwal (2003) Biochem. Pharmacol. 66:1403.
  3. Collette, Y. et al. (2003) Trends Immunol. 24:387.
  4. Mizuno, T. et al. (1998) Vet. Immunol. Immunopathol. 65:161.
  5. Mizuno, T. et al. (2004) Eur. J. Immunogenet. 31:159.
  6. Knipping, E. et al. (1995) Blood 85:1562.
  7. Baker, S.J. and E.P. Reddy (1998) Oncogene 17:3261.
Long Name
Entrez Gene IDs
355 (Human); 14102 (Mouse); 246097 (Rat); 102140989 (Cynomolgus Monkey); 493881 (Feline)
Alternate Names
Apo-1 antigen; Apo-1; apoptosis antigen 1; Apoptosis-mediating surface antigen FAS; APT1; APT1FASTM; CD95 antigen; CD95; CD95ALPS1A; Fas (TNF receptor superfamily, member 6); Fas AMA; Fas antigen; Fas; FAS1; FASLG receptor; TNFRSF6; TNFRSF6member 6; tumor necrosis factor receptor superfamily member 6

Product Datasheets

Product Specific Notices

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.


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