FGF-12 is a member of the FGF superfamily of heparin-binding mitogenic molecules characterized by the presence of a core, 120 amino acid (aa) beta-trefoil structure. FGF-11, -12, -13, -14, originally termed FGF homologous factors (FHF) -3, -1, -2, and -4, respectively, form a subgroup within the FGF family. Human FGF-12/FHF-1 is synthesized as a 243 aa protein. It lacks a typical signal sequence and is considered cytoplasmic. It also possess an N-terminal bipartite nuclear localization signal at aa 11-18 and 28-38.
FGF-12 Antibody [DyLight 350]
Novus Biologicals | Catalog # AF2246D
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
DyLight 350 (Excitation = 353 nm, Emission = 432 nm)
Antibody Source
Polyclonal Goat IgG
Loading...
Product Specifications
Immunogen
E. coli-derived recombinant human FGF-12
Glu67-Thr243
Accession # P61328
Glu67-Thr243
Accession # P61328
Specificity
Detects human FGF-12 in direct ELISAs and Western blots. In direct ELISAs, approximately 35% cross-reactivity with recombinant human (rh) FGF-13B is observed, 10% cross-reactivity with rhFGF-11 is observed and less than 1% cross-reactivity with rhFGF acidic, rhFGF basic, rhFGF-3, rhFGF-4, rhFGF-6, rhFGF-7, rhFGF-9, rhFGF-10, rhFGF-16, rhFGF-17, rhFGF-18, rhFGF-19, rhFGF-20, rhFGF-23, recombinant mouse (rm) FGF-15, and rmFGF-8b is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for FGF-12 Antibody [DyLight 350]
Application
Recommended Usage
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: FGF-12
Long Name
Fibroblast Growth Factor 12
Alternate Names
FGF12, FHF1
Gene Symbol
FGF12
Additional FGF-12 Products
Product Documents for FGF-12 Antibody [DyLight 350]
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for FGF-12 Antibody [DyLight 350]
DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Customer Reviews for FGF-12 Antibody [DyLight 350]
There are currently no reviews for this product. Be the first to review FGF-12 Antibody [DyLight 350] and earn rewards!
Have you used FGF-12 Antibody [DyLight 350]?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...