FGF activity is mediated by a family of type I transmembrane tyrosine kinases, which undergo dimerization and autophosphorylation after ligand binding. Five distinct genes encode closely related FGF receptors, FGFR1 through 5. FGFRs contain three Ig-like domains and a stretch of acidic residues between the first and second Ig-like domains. FGFR1, 2, 3, and -4 have a cytoplasmic split tyrosine-kinase domain, but FGFR5 does not. Multiple forms of FGFR1, 2, and 3 are generated by alternative splicing.
Human FGFR2 ELISA Kit (Colorimetric)
Novus Biologicals | Catalog # NBP2-80324
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Key Product Details
Sample Type & Volume Required Per Well
Recombinant Protein (100 ul)
Sensitivity
7.15 pg/ml
Assay Range
10.94-700 pg/ml (example only; lot dependent)
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Product Specifications
Assay Type
Sandwich ELISA
Kit Type
ELISA Kit (Colorimetric)
Conjugate
HRP
Species
Human
Specificity
Recognizes both recombinant and natural Human FGFR2.
Precision
Intra-Assay Precision (Precision within an assay) CV% 6.13
Inter-Assay Precision (Precision between assays) CV% 7.6
Recovery for Human FGFR2 ELISA Kit (Colorimetric)
Recovery
serum (n=3): 81 -87%
supernatant (n=3): 81 -87%
urine (n=3): 81 -87%
supernatant (n=3): 81 -87%
urine (n=3): 81 -87%
Scientific Data Images for Human FGFR2 ELISA Kit (Colorimetric)
ELISA: Human FGFR2 ELISA Kit (Colorimetric) [NBP2-80324]
ELISA: Human FGFR2 ELISA Kit (Colorimetric) [NBP2-80324] - Example standard curve for Human FGFR2 ELISA Kit (Colorimetric). These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.Kit Contents for Human FGFR2 ELISA Kit (Colorimetric)
- 1. 96 well microplate coated with Capture Antibody
- 2. Detection Antibody conjugated to HRP
- 3. Standards
- 4. Wash Buffer Concentrate
- 5. Dilution Buffer Concentrate
- 6. Color Reagent A
- 7. Color Reagent B
- 8. Stop Solution
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Storage is content dependent.
Background: FGFR2
Long Name
Fibroblast Growth Factor Receptor 2
Alternate Names
CD332, FGF R2
Gene Symbol
FGFR2
Additional FGFR2 Products
Product Documents for Human FGFR2 ELISA Kit (Colorimetric)
Product Specific Notices for Human FGFR2 ELISA Kit (Colorimetric)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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