FGFR4 Antibody (DMC460) - Chimeric

Novus Biologicals | Catalog # NBP3-48345

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Human IgG1 Clone # DMC460
View all FGFR4 Primary Antibodies »

Product Specifications

Immunogen

FGFR4 recombinant protein expressed in mammalian cells (Accession# P22455). The exact amino acid sequence is proprietary.

Clonality

Monoclonal

Host

Human

Isotype

IgG1

Description

This product is shipped at ambient temperature. Store at -20C to -80C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80C (Avoid repeated freezing and thawing).

Scientific Data Images for FGFR4 Antibody (DMC460) - Chimeric

FGFR4 Antibody (DMC460) - Chimeric

Flow Cytometry: FGFR4 Antibody (DMC460) - Chimeric [NBP3-48345]

Flow cytometry analysis of antigen binding of anti-human FGFR4 mAb(NBP3-48345). (A) NBP3-48345 does not bind to SiHa cells that do not express FGFR4. (B) A clear peak shift of NBP3-48345 was seen compared to the control when incubated with FGFR4-expressing Huh7 cells, indicating strong binding of NBP3-48345 to FGFR4. Antibodies were incubated at 5 ug/mL.
FGFR4 Antibody (DMC460) - Chimeric

Flow Cytometry: FGFR4 Antibody (DMC460) - Chimeric [NBP3-48345]

Flow cytometry analysis with Anti-FGFR4 (DMC460) on HEK293 cells transfected with human FGFR4 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram).

Applications for FGFR4 Antibody (DMC460) - Chimeric

Application
Recommended Usage

Flow Cytometry

1:100

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified from cell culture supernatant

Reconstitution

Dissolve the lyophilized antibody in sterile and low endotoxin water. The recommend volume of water is lot specific. Please refer to the instructions printed on the product vial or contact technical support.

Formulation

PBS, pH 7.4, 5-8% Trehalose

Preservative

Trehalose

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C in powder form. Store at -80C once reconstituted.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FGFR4

FGF activity is mediated by a family of type I transmembrane tyrosine kinases, which undergo dimerization and autophosphorylation after ligand binding. Five distinct genes encode closely related FGF receptors, FGFR1 through 5. FGFRs contain three Ig-like domains and a stretch of acidic residues between the first and second Ig-like domains. FGFR1, 2, 3, and -4 have a cytoplasmic split tyrosine-kinase domain, but FGFR5 does not. Multiple forms of FGFR1, 2, and 3 are generated by alternative splicing.

Long Name

Fibroblast Growth Factor Receptor 4

Alternate Names

CD334, FGF R4

Gene Symbol

FGFR4

Additional FGFR4 Products

Product Documents for FGFR4 Antibody (DMC460) - Chimeric

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for FGFR4 Antibody (DMC460) - Chimeric

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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