FGL2 (fibrinogen-like protein 2), also called fibroleukin, is a 64-70 kDa secreted glycoprotein of the Fibrinogen-like superfamily. In the presence of acidic phospholipids and calcium, FGL2 has prothrombinase activity and acts as a procoagulant. In soluble form, FGL2 promotes T regulatory (Treg) activity and suppresses DC, T, and B cell immune reactivity, acting as an immune suppressor. A 260-280 aa FGL2 complex is thought to be a homotetramer.
FGL2/Fibroleukin Overexpression Lysate
Novus Biologicals | Catalog # NBP2-10401
![Western Blot: FGL2/Fibroleukin Overexpression Lysate [NBP2-10401]](https://resources.rndsystems.com/images/products/Fibroleukin-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-10401-img0001.jpg "Western Blot: FGL2/Fibroleukin Overexpression Lysate [NBP2-10401]")
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Key Product Details
Species
Human
Applications
Western Blot
Product Summary for FGL2/Fibroleukin Overexpression Lysate
FGL2/Fibroleukin Transient Overexpression Lysate
Expression Host: HEK293T
Plasmid: RC207557
Accession#: NM_006682
Protein Tag: C-MYC/DDK
You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
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Product Specifications
Application Notes
This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.
Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
47.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Type
Overexpression
Scientific Data Images for FGL2/Fibroleukin Overexpression Lysate
Western Blot: FGL2/Fibroleukin Overexpression Lysate [NBP2-10401]
Western Blot: Fibroleukin Overexpression Lysate (Adult Normal) [NBP2-10401] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Fibroleukin.Formulation, Preparation, and Storage
Formulation
RIPA buffer
Concentration
The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.
Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store at -80C. Avoid freeze-thaw cycles.
Background: FGL2
Long Name
Fibrinogen-like 2
Alternate Names
Fibroleukin, T49
Gene Symbol
FGL2
Additional FGL2 Products
Product Documents for FGL2/Fibroleukin Overexpression Lysate
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for FGL2/Fibroleukin Overexpression Lysate
HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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