Fibronectin Antibody (25A1) - BSA Free
Novus Biologicals | Catalog # NBP3-26576
Key Product Details
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Scientific Data Images for Fibronectin Antibody (25A1) - BSA Free
Flow Cytometry: Fibronectin Antibody (25A1) [NBP3-26576] -
Flow Cytometry: Fibronectin Antibody (25A1) [NBP3-26576] - Overlay Peak curve showing 3T3 cells stained with Fibronectin Antibody (25A1) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.Applications for Fibronectin Antibody (25A1) - BSA Free
Flow Cytometry
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
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Background: Fibronectin
Alternate Names
Gene Symbol
Additional Fibronectin Products
Product Documents for Fibronectin Antibody (25A1) - BSA Free
Certificate of Analysis
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Product Specific Notices for Fibronectin Antibody (25A1) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
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FAQs for Fibronectin Antibody (25A1) - BSA Free
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Q: What is the difference between Bovine Fibronectin Protein, CF (Catalog # 1030-FN) and Human Fibronectin Protein, CF (Catalog # 1918-FN)?
A: Only the source species for the protein is different. Both carrier-free proteins have been validated to be bioactive with the same assay protocols listed and recommended concentrations fall within the same range, although customers should determine their optimal concentrations for their particular cell type and application.
