FITC Antibody (LO-FLUO-1) - BSA Free

Novus Biologicals | Catalog # NB100-63003

Novus Biologicals
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Key Product Details

Species Reactivity

Non-species specific

Applications

ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgM Clone # LO-FLUO-1

Format

BSA Free
View all FITC Primary Antibodies »

Product Specifications

Immunogen

FITC conjugated mouse immunoglobulin

Specificity

Binds to both free FITC and FITC conjugated to protein such as antibodies. The reagent may be useful for amplification of staining using FITC conjugated reagents.

Clonality

Monoclonal

Host

Rat

Isotype

IgM

Applications for FITC Antibody (LO-FLUO-1) - BSA Free

Application
Recommended Usage

ELISA

1.25 ug/ml-2.25 ug/ml

Flow Cytometry

1:10-1:1000

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

IgM purified

Formulation

PBS

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FITC

FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins.

Long Name

Fluorescein Isothiocyanate

Alternate Names

Fluorescein Isothiocyanate

Additional FITC Products

Product Documents for FITC Antibody (LO-FLUO-1) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for FITC Antibody (LO-FLUO-1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for FITC Antibody (LO-FLUO-1) - BSA Free

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  • Q: What is the molecular weight of Fluorescein Isothiocyanate (FITC)?

    A: The molecular weight of FITC is 389.38 Daltons. However, R&D Systems uses 5- (and 6-) Carboxyfluorescein (CFS) for antibody conjugation, which has a molecular weight of 473 Daltons.

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