FK506 binding protein, 25 kDa molecular weight (FKBP25), also called FKBP3, is a peptidyl-prolyl isomerase that catalyzes the transition between cis- and trans-proline residues critical for proper folding of proteins. The macrolide immunosuppressant Rapamycin is a potent inhibitor of FKBP25. FKBP25 is localized in the nucleus, where it alters the activity of histone deacetylases and transcription factors. FKBP25 is found in many tissues, including the brain, thymus, and spleen.
FKBP25 Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-87929
![FKBP25 Antibody - BSA Free Immunocytochemistry/ Immunofluorescence: FKBP25 Antibody - BSA Free [NBP1-87929]](https://resources.rndsystems.com/images/products/nbp1-87929_rabbit-polyclonal-fkbp25-antibody-65202516213814.jpg "Immunocytochemistry/ Immunofluorescence: FKBP25 Antibody - BSA Free [NBP1-87929]")
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Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: LPKKDIIKFLQEHGSDSFLAEHKLLGNIKNVAKTANKDHLVTAYNHLFETKRFKGTESISKVSEQVKNVKLNEDKPKETKSEETLDEGPPKYTKSVLKKGDKTNFPKKGDVVHCWYTGTLQDGT
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for FKBP25 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence: FKBP25 Antibody - BSA Free [NBP1-87929]
Staining of human cell line U-2 OS shows localization to cytosol.![Immunohistochemistry-Paraffin: FKBP25 Antibody [NBP1-87929]](https://resources.rndsystems.com/images/products/FKBP25-Antibody-Immunohistochemistry-Paraffin-NBP1-87929-img0008.jpg "Immunohistochemistry-Paraffin: FKBP25 Antibody [NBP1-87929]")
Immunohistochemistry-Paraffin: FKBP25 Antibody [NBP1-87929]
Immunohistochemistry-Paraffin: FKBP25 Antibody [NBP1-87929] - Staining of human testis shows moderate to strong cytoplasmic positivity in cells in seminiferous ducts.![Immunohistochemistry-Paraffin: FKBP25 Antibody [NBP1-87929]](https://resources.rndsystems.com/images/products/FKBP25-Antibody-Immunohistochemistry-Paraffin-NBP1-87929-img0006.jpg "Immunohistochemistry-Paraffin: FKBP25 Antibody [NBP1-87929]")
Immunohistochemistry-Paraffin: FKBP25 Antibody [NBP1-87929]
Immunohistochemistry-Paraffin: FKBP25 Antibody [NBP1-87929] - Staining of human cerebral cortex shows moderate to strong cytoplasmic positivity in neurons.![Immunohistochemistry-Paraffin: FKBP25 Antibody [NBP1-87929]](https://resources.rndsystems.com/images/products/FKBP25-Antibody-Immunohistochemistry-Paraffin-NBP1-87929-img0007.jpg "Immunohistochemistry-Paraffin: FKBP25 Antibody [NBP1-87929]")
Immunohistochemistry-Paraffin: FKBP25 Antibody [NBP1-87929]
Immunohistochemistry-Paraffin: FKBP25 Antibody [NBP1-87929] - Staining of human lymphoid tissues shows moderate to strong cytoplasmic positivity in germinal center cells.![Immunohistochemistry-Paraffin: FKBP25 Antibody [NBP1-87929]](https://resources.rndsystems.com/images/products/FKBP25-Antibody-Immunohistochemistry-Paraffin-NBP1-87929-img0009.jpg "Immunohistochemistry-Paraffin: FKBP25 Antibody [NBP1-87929]")
Immunohistochemistry-Paraffin: FKBP25 Antibody [NBP1-87929]
Immunohistochemistry-Paraffin: FKBP25 Antibody [NBP1-87929] - Staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.![FKBP25 Antibody - BSA Free Western Blot: FKBP25 Antibody - BSA Free [NBP1-87929]](https://resources.rndsystems.com/images/products/nbp1-87929_rabbit-polyclonal-fkbp25-antibody-65202516262611.jpg "Western Blot: FKBP25 Antibody - BSA Free [NBP1-87929]")
Western Blot: FKBP25 Antibody - BSA Free [NBP1-87929]
Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
Lane 3: PC12 cell lysate (Pheochromocytoma of rat adrenal medulla)
![FKBP25 Antibody - BSA Free Western Blot: FKBP25 Antibody - BSA Free [NBP1-87929]](https://resources.rndsystems.com/images/products/nbp1-87929_rabbit-polyclonal-fkbp25-antibody-65202516264323.jpg "Western Blot: FKBP25 Antibody - BSA Free [NBP1-87929]")
Western Blot: FKBP25 Antibody - BSA Free [NBP1-87929]
Lane 1: Marker [kDa] 230, 110, 82, 49, 32, 26, 18Lane 2: Human cell line RT-4
Lane 3: Human cell line U-251MG sp
Applications for FKBP25 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: FKBP25
Long Name
25 kDa FK506 Binding Protein
Alternate Names
FKBP3
Gene Symbol
FKBP3
Additional FKBP25 Products
Product Documents for FKBP25 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for FKBP25 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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