FKBP25 Antibody

Western Blot: FKBP25 Antibody [NBP3-18658]

Western Blot: FKBP25 Antibody [NBP3-18658] - Figure 2. Western blot analysis using the antibody directed against FKBP25 (1) Western blot was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) with the antibody against mouse FKBP25, diluted 1:500 in BSA/ PBS-Tween. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest (expected size: 25 kDa) is indicated on the right.

ELISA: FKBP25 Antibody [NBP3-18658]

ELISA: FKBP25 Antibody [NBP3-18658] - Figure 1. Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against mouse FKBP25. The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:5,400.