Flagellin Native Protein

Name: Flagellin Native Protein
Brand: Novus Biologicals
SKU: NBP3-18149
Price: 239 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-18149

Submit a review

Product Documents

100% Guarantee

Product Specifications
Scientific Data
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices

Key Product Details

Accession Number

P06179

Applications

ELISA, Bioactivity

View all Flagellin Proteins and Enzymes »

Product Specifications

Description

Source: Salmonella typhimurium strain 14028

Uniprot ID: P06179

Purity

>95% pure by SDS-PAGE

Endotoxin Level

<0.01EU/ug purified protein (LAL test; Lonza).

Activity

Binds to human & mouse TLR5. Activation of TLR5 in human epithelial cell assays based on NF-kB luciferase fusions, on expression of IL-8 (CXCL8), CCL20, inducible nitric oxide synthase, beta-defensin 2 & matrilysin (MMP-7). Maturation of dendritic cells in in vitro & in vivo assays. Adjuvant activity on foreign antigens delivered by subcutaneous & intranasal routes in mice. TLR5 can be stimulated in vitro by Flagellin (high Purity) at 5-50ng/ml & in vivo at a starting concentration of 0.1-1ug /mouse.

Specificity

Binds to human and mouse TLR5 (Toll-like receptor 5).

Protein / Peptide Type

Native Protein

Scientific Data Images for Flagellin Native Protein

ELISA: Flagellin Native Protein [NBP3-18149]

ELISA: Flagellin Native Protein [NBP3-18149] - Flagellin activates TLR5 in vivo.Method: Mice WT are injected i.v. with indicated doses of Flagellin. After 2 hours, levels of CCL20 and IL-6 in the serum were measured by ELISA. As a control, mice TLR5 -/- have been injected i.v. with 10ug of Flagellin, but no expression of CCL20 and IL-6 could be observed (not shown).

Formulation, Preparation, and Storage

NBP3-18149

Formulation	Lyophilized from PBS
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Reconstitute with 100 ul sterile water (0.1mg/ml after reconstitution)
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: Flagellin

UniProt

P06179 (Bacteria)

Additional Flagellin Products

Product Documents for Flagellin Native Protein

Download Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Flagellin Native Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Customer Reviews for Flagellin Native Protein

There are currently no reviews for this product. Be the first to review Flagellin Native Protein and earn rewards!

Have you used Flagellin Native Protein?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image