Recombinant Bacteria Flagellin NLRC4 Mutant His Protein
Novus Biologicals | Catalog # NBP3-18158
![Functional: Recombinant Bacteria Flagellin NLRC4 Mutant His Protein [NBP3-18158]](https://resources.rndsystems.com/images/products/Recombinant-Human--Mouse-Flagellin-NLRC4-Mutant-His-Protein-Functional-NBP3-18158-img0001.jpg "Functional: Recombinant Bacteria Flagellin NLRC4 Mutant His Protein [NBP3-18158]")
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Key Product Details
Source
E. coli
Accession Number
Tag
His
Applications
Bioactivity
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Product Specifications
Description
Native flagellin [FliC] from Salmonella enterica Serovar Typhimurium strain ATCC14028 (aa 1-495) contains a small deletion at the C-terminus of the protein required for NLRC4 activation and fused at the N-terminus to a His-tag.
Source: E. coli
Source: E. coli
NCBI: NP_460912
Purity
>95% pure by SDS-PAGE
Endotoxin Level
<0.01EU/ug purified protein (LAL test; Lonza).
Activity
Detected by human & mouse TLR5. Not detected by mouse NLRC4. Activation of TLR5 in human epithelial cell assays based on NF-kB luciferase fusions, in vivo on expression of CCL20 or IL-6. TLR5 can be stimulated in vitro by Flagellin (NLRC4 mutant) (rec.) at 5-50ng/ml & in vivo at a starting concentration of 0.1-1 ug/mouse. Does not activate NLRC4 as tested in bone-marrow mouse macrophages (in the presence of streptolysin O (SLO)). It can be used as negative control for NLRC4 signaling.
Specificity
Detected by human and mouse TLR5 (Toll-like receptor 5). Not detected by mouse NLRC4 (Nod-like receptor [NLR] family caspase activation and recruitment domain [CARD] domain-containing protein 4).
Protein / Peptide Type
Recombinant Protein
Scientific Data Images for Recombinant Bacteria Flagellin NLRC4 Mutant His Protein
Functional: Recombinant Bacteria Flagellin NLRC4 Mutant His Protein [NBP3-18158]
Functional: Recombinant Human, Mouse Flagellin NLRC4 Mutant His Protein [NBP3-18158] - Flagellin (rec.) and Flagellin (NLRC4 Mutant) (rec.)induce CCL20-luciferase promoter through TLR5 in vitro.Method: Caco-2 cells transfected with plasmid encoding CCL20-LUC plasmid are activated for 24h with the indicated concentrations of Flagellin (rec.)and (NLRC4 Mutant) (rec.). As a negative control, Flagellin (TLR5 Mutant) (rec.) is used. Luciferase assays are performed on cell lysates and luminescence is normalized to mock-treated cells.Formulation, Preparation, and Storage
NBP3-18158
| Formulation | Lyophilized from PBS |
| Preservative | No Preservative |
| Concentration | Lyoph |
| Reconstitution | Reconstitute with 100 ul sterile water (0.1mg/ml after reconstitution) |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Calculators
Background: Flagellin
UniProt
Additional Flagellin Products
Product Documents for Recombinant Bacteria Flagellin NLRC4 Mutant His Protein
Certificate of Analysis
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Product Specific Notices for Recombinant Bacteria Flagellin NLRC4 Mutant His Protein
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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