FLAP Antibody

Novus Biologicals | Catalog # NB100-53797

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Canine (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Flap Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-HKVEHESRTQNGR corresponding to internal region according to NP_001620.2.

Epitope

C-HKVEHESRTQNGR

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for FLAP Antibody

Western Blot: FLAP Antibody [NB100-53797]

Western Blot: FLAP Antibody [NB100-53797]

Western Blot: FLAP Antibody [NB100-53797] - Human U937 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: FLAP Antibody [NB100-53797]

Immunohistochemistry-Paraffin: FLAP Antibody [NB100-53797]

Immunohistochemistry-Paraffin: FLAP Antibody [NB100-53797] - (3.8ug/ml) staining of Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for FLAP Antibody

Application
Recommended Usage

Immunohistochemistry

3 - 6 ug/ml

Immunohistochemistry-Paraffin

3 - 6 ug/ml

Peptide ELISA

Detection limit 1:128000

Western Blot

0.1 - 0.3 ug/ml
Application Notes
WB: Approx. 16 kDa band observed in lysates of U937 and of peripheral blood mononucleocytes (calculated MW of 18.2 kDa band according to NP_001620.2). IHC-P: Human spleen shows staining of select splenocytes.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Flap

This gene encodes a protein which, with 5-lipoxygenase, is required for leukotriene synthesis. Leukotrienes are arachidonic acid metabolites which have been implicated in various types of inflammatory responses, including asthma, arthritis and psoriasis. This protein localizes to the plasma membrane. Inhibitors of its function impede translocation of 5-lipoxygenase from the cytoplasm to the cell membrane and inhibit 5-lipoxygenase activation.

Alternate Names

arachidonate 5-lipoxygenase-activating protein, FLAPMK-886-binding protein

Entrez Gene IDs

241 (Human)

Gene Symbol

ALOX5AP

UniProt

NP_001620.2 (Human)

Additional Flap Products

Product Documents for FLAP Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for FLAP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Base Excision Repair

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Protocols

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FAQs

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