FLASH Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-77301

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (94%). Backed by our 100% Guarantee.

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all FLASH Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a synthetic peptide corresponding to amino acids near the carboxy terminus of human FLASH which differ from those of mouse by one amino acid. The immunogen is located within the last 50 amino acids of FLASH. Amino Acid Squence: SERFQQLMKLFEKSKC

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

220 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for FLASH Antibody - BSA Free

Western Blot: FLASH AntibodyBSA Free [NBP1-77301]

Western Blot: FLASH AntibodyBSA Free [NBP1-77301]

Western Blot: FLASH Antibody [NBP1-77301] - Analysis of FLASH in HeLa whole cell lysate with FLASH antibody at 0.5 ug/ml.
FLASH Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: FLASH Antibody - BSA Free [NBP1-77301] -

Immunocytochemistry/ Immunofluorescence: FLASH Antibody - BSA Free [NBP1-77301] - Immunocytochemistry of FLASH in HeLa cells with FLASH antibody at 10 u/mL.
FLASH Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: FLASH Antibody - BSA Free [NBP1-77301] -

Immunocytochemistry/ Immunofluorescence: FLASH Antibody - BSA Free [NBP1-77301] - Immunofluorescence of FLASH in HeLa cells with FLASH antibody at 10 ug/mL.

Applications for FLASH Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Western Blot

0.5 ug/ml
Application Notes
An approximate 220 kDa band can be detected in Western Blot.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FLASH

A novel mammalian CED-4 homologous was recently identified and cloned in mouse and human and designated FLASH (for FLICE-associated huge protein). FLASH is involved in Fas induced apoptosis. It is recruited to Fas after the receptor cross-linking. Overexpression of wild type of FLASH facilitates and its dominant negative form inhibits Fas induced apoptosis. FLASH interacts with the DEDs of caspase-8 and FADD through the DED-like domain of FLASH and mediates activation of caspase-8. There are parallels between FLASH and Apaf-1/CED-4 although there are arguments against their structural similarity. FLASH is widely expressed.

Alternate Names

CASP8 associated protein 2, caspase 8 associated protein 2, CED-4, FLASHKIAA1315CASP8-associated protein 2, FLICE associated huge, FLICE-associated huge protein, FLJ11208, human FLASH, RIP25FLASH homolog RIP25

Gene Symbol

CASP8AP2

UniProt

AF165161 (Human)

Additional FLASH Products

Product Documents for FLASH Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for FLASH Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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