Flotillin-1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-87498

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Flotillin-1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: HQRAIMAHMTVEEIYKDRQKFSEQVFKVASSDLVNMGISVVSYTLKDIHDDQDYLHSLGKARTAQVQKDARIGEAEAKRDAGIREAKAKQEKVSAQYLSEIEMAKAQRDYELKKAAYDIEVNTRRAQADLAYQLQVAKTKQQIEEQR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Flotillin-1 Antibody - BSA Free

Flotillin-1 Antibody

Immunohistochemistry-Paraffin: Flotillin-1 Antibody [NBP1-87498] -

Immunohistochemistry-Paraffin: Flotillin-1 Antibody [NBP1-87498] -Analysis in human adrenal gland and skin tissues using NBP1-87498 antibody. Corresponding FLOT1 RNA-seq data are presented for the same tissues.
Western Blot: Flotillin-1 Antibody [NBP1-87498]

Western Blot: Flotillin-1 Antibody [NBP1-87498]

Western Blot: Flotillin-1 Antibody [NBP1-87498] - Analysis in human cell lines SK-MEL-30 and MCF-7 using Anti-FLOT1 antibody. Corresponding FLOT1 RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.
Western Blot: Flotillin-1 Antibody [NBP1-87498]

Western Blot: Flotillin-1 Antibody [NBP1-87498]

Western Blot: Flotillin-1 Antibody [NBP1-87498] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunohistochemistry-Paraffin: Flotillin-1 Antibody [NBP1-87498]

Immunohistochemistry-Paraffin: Flotillin-1 Antibody [NBP1-87498]

Immunohistochemistry-Paraffin: Flotillin-1 Antibody [NBP1-87498] - Staining of human adrenal gland shows high expression.
Flotillin-1 Antibody

Immunohistochemistry-Paraffin: Flotillin-1 Antibody [NBP1-87498] -

Immunohistochemistry-Paraffin: Flotillin-1 Antibody [NBP1-87498] -Staining of human Adrenal gland shows strong membranous positivity in glandular cells.
Flotillin-1 Antibody

Immunohistochemistry-Paraffin: Flotillin-1 Antibody [NBP1-87498] -

Immunohistochemistry-Paraffin: Flotillin-1 Antibody [NBP1-87498] - Staining of human Fallopian tube shows strong membranous positivity in glandular cells.
Flotillin-1 Antibody

Immunohistochemistry-Paraffin: Flotillin-1 Antibody [NBP1-87498] -

Immunohistochemistry-Paraffin: Flotillin-1 Antibody [NBP1-87498] - Staining of human Stomach shows strong membranous positivity in glandular cells.
Flotillin-1 Antibody

Immunohistochemistry-Paraffin: Flotillin-1 Antibody [NBP1-87498] -

Immunohistochemistry-Paraffin: Flotillin-1 Antibody [NBP1-87498] -Staining of human Skin shows very weak membranous positivity in squamous epithelial cells.

Applications for Flotillin-1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Flotillin-1

Caveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction. FLOT1 encodes a caveolae-associated, integral membrane protein. The function of flotillin 1 has not been determined.

Alternate Names

flotillin 1, flotillin-1, integral membrane component of caveolae

Gene Symbol

FLOT1

Additional Flotillin-1 Products

Product Documents for Flotillin-1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Flotillin-1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Flotillin-1 Antibody - BSA Free

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Protocols

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