Flp recombinase Antibody

Novus Biologicals | Catalog # NBP3-11867

Novus Biologicals
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Key Product Details

Species Reactivity

Non-species specific

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit
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Product Specifications

Immunogen

Polyclonal antibody raised in rabbit against Flp recombinase using 3 KLH-conjugated synthetic peptides located at the N-terminal part of the protein (Uniprot#: P03870)

Clonality

Polyclonal

Host

Rabbit

Scientific Data Images for Flp recombinase Antibody

Western Blot: Flp recombinase Antibody [NBP3-11867]

Western Blot: Flp recombinase Antibody [NBP3-11867]

Western Blot: Flp recombinase Antibody [NBP3-11867] - Western blot was performed on whole cell lysates from untransfected 293 cells (lane 1), or 293 cells transfected with Cre (lane 2), Dre (lane 3) or Flp (lane 4) with NBP3-11867 diluted 1:500 in BSA/PBS-Tween. The molecular weight marker (in KD) is shown on the left; the location of the protein of interest (expected size: 48 KD) is indicated on the right.
ELISA: Flp recombinase Antibody [NBP3-11867]

ELISA: Flp recombinase Antibody [NBP3-11867]

ELISA: Flp recombinase Antibody [NBP3-11867] - To determine the titer, an ELISA was performed using a serial dilution of NBP3-11867 in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:9,000.

Applications for Flp recombinase Antibody

Application
Recommended Usage

ELISA

1:500

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

Whole Antiserum

Formulation

Whole rabbit antiserum

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: Flp recombinase

Flp recombinase originates from Saccharomyces cerevisiae and recognizes FRT sites in the genome. It catalyzes recombination between two FRT sites, thereby excising the intervening DNA sequence. Flp recombinase is used as a tool for the generation of transgenic animals.

Additional Flp recombinase Products

Product Documents for Flp recombinase Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Flp recombinase Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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