Folate Receptor 2 (FOLR2), also known as Folate Receptor beta, is a 38 kDa glycoprotein that is anchored to the membrane via glycosyl phosphatidylinositol. It mediates the cellular uptake of dietary folates that are required for key steps in amino acid metabolism. FOLR2 is predominantly expressed in placenta, myeloid cells, and some CD34+ hematopoietic progenitor cells. It is upregulated on some cancer cells and at sites of chronic inflammation.
FOLR2 Antibody - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-99741
![Western Blot: FOLR2 Antibody [NBP2-99741]](https://resources.rndsystems.com/images/products/FOLR2-Antibody-Western-Blot-NBP2-99741-img0004.jpg "Western Blot: FOLR2 Antibody [NBP2-99741]")
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
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Product Specifications
Immunogen
Produced in rabbits immunized with purified, recombinant Human FOLR2 (Accession#: P14207-1; Met1-His228)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for FOLR2 Antibody - Azide and BSA Free
Western Blot: FOLR2 Antibody [NBP2-99741]
Western Blot: FOLR2 Antibody [NBP2-99741] - Anti-FOLR2 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lane B: MCF-7 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 29 kDa. Observed band size: 29 kDa (We are unsure as to the identity of these extra bands).![Immunohistochemistry-Paraffin: FOLR2 Antibody [NBP2-99741]](https://resources.rndsystems.com/images/products/FOLR2-Antibody-Immunohistochemistry-Paraffin-NBP2-99741-img0003.jpg "Immunohistochemistry-Paraffin: FOLR2 Antibody [NBP2-99741]")
Immunohistochemistry-Paraffin: FOLR2 Antibody [NBP2-99741]
Immunohistochemistry-Paraffin: FOLR2 Antibody [NBP2-99741] - Immunochemical staining of human FOLR2 in human spleen with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.![Immunohistochemistry-Paraffin: FOLR2 Antibody [NBP2-99741]](https://resources.rndsystems.com/images/products/FOLR2-Antibody-Immunohistochemistry-Paraffin-NBP2-99741-img0002.jpg "Immunohistochemistry-Paraffin: FOLR2 Antibody [NBP2-99741]")
Immunohistochemistry-Paraffin: FOLR2 Antibody [NBP2-99741]
Immunohistochemistry-Paraffin: FOLR2 Antibody [NBP2-99741] - Immunochemical staining of human FOLR2 in human placenta with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.Applications for FOLR2 Antibody - Azide and BSA Free
Application
Recommended Usage
ELISA
1:5000-1:10000
Immunohistochemistry
1:500-1:2000
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
0.2 um filtered solution in PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: FOLR2
Long Name
Folate Receptor 2
Alternate Names
beta-HFR, FR-beta, FR-P3
Gene Symbol
FOLR2
Additional FOLR2 Products
Product Documents for FOLR2 Antibody - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for FOLR2 Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for FOLR2 Antibody - Azide and BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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