FUBP1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-16542

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (99%), Chicken (94%), Xenopus (92%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all FUBP1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human FUBP1. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (92%), Zebrafish (83%).

Localization

Nucleus

Marker

Nucleus without Nucleoli Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for FUBP1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: FUBP1 Antibody [NBP2-16542]

Immunocytochemistry/ Immunofluorescence: FUBP1 Antibody [NBP2-16542]

Immunocytochemistry/Immunofluorescence: FUBP1 Antibody [NBP2-16542] - Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FUBP1 protein stained by FUBP1 antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: FUBP1 Antibody [NBP2-16542]

Immunohistochemistry-Paraffin: FUBP1 Antibody [NBP2-16542]

Immunohistochemistry-Paraffin: FUBP1 Antibody [NBP2-16542] - Mouse testis. FUBP1 antibody dilution: 1:1000. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: FUBP1 Antibody [NBP2-16542]

Western Blot: FUBP1 Antibody [NBP2-16542]

Western Blot: FUBP1 Antibody [NBP2-16542] - 293T whole cell lysate/extract A : 30 ug whole cell lysate/extract of FUBP-1 protein expressing 293T cells B : Control with 2.5 ug of pre-immune rabbit IgG C : Immunoprecipitation of FUBP-1 by 2.5 ug of FUBP-1 antibody 7.5% SDS-PAGE The immunoprecipitated FUBP-1 protein was detected by FUBP-1 antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.
Western Blot: FUBP1 Antibody [NBP2-16542]

Western Blot: FUBP1 Antibody [NBP2-16542]

Western Blot: FUBP1 Antibody [NBP2-16542] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FUBP1 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: FUBP1 Antibody [NBP2-16542]

Immunohistochemistry-Paraffin: FUBP1 Antibody [NBP2-16542]

Immunohistochemistry-Paraffin: FUBP1 Antibody [NBP2-16542] - Mouse testis. FUBP1 antibody dilution: 1:1000. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
FUBP1 Antibody - BSA Free

Western Blot: FUBP1 Antibody [NBP2-16542] -

HeLa whole cell and nuclear extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FUBP1 antibody (NBP2-16542) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
FUBP1 Antibody - BSA Free

Western Blot: FUBP1 Antibody [NBP2-16542] -

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FUBP1 antibody (NBP2-16542) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for FUBP1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:1000-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: FUBP1

This gene encodes a ssDNA binding protein that activates the far upstream element (FUSE) of c-myc and stimulates expression of c-myc in undifferentiated cells. Regulation of FUSE by FUBP occurs through single-strand binding of FUBP to the non-coding strand. This protein has been shown to function as an ATP-dependent DNA helicase. [provided by RefSeq]

Alternate Names

far upstream element (FUSE) binding protein 1, far upstream element binding protein, far upstream element-binding protein 1, FBPDNA helicase V, FUBP, FUSE-binding protein 1, hDH V

Gene Symbol

FUBP1

UniProt

Q96AE4 (Human)

Additional FUBP1 Products

Product Documents for FUBP1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for FUBP1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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FAQs

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