Detection of GABAA R beta 2 C-Terminus by Western Blot. Western blot of 5 - 7 μg rat brain (cerebellum) lysates. The blot was incubated overnight at 2 - 8° C with anti-GABAA R beta 2 subunit, C-Terminus diluted 1:1000. The antibody labeled the ~50 - 56 kDa beta 2 subunit of the GABAA R.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.
Background: GABA-A R beta 2
GABAA ( gamma -aminobutyric acid-type A) receptors are members of the cysteine-loop family of neurotransmitter-gated ion channels. GABA binding to A-type receptors induces anion-selective ion channel opening. These receptors are the principal fast inhibitory neurotransmitter receptors in the CNS. GABAA receptors are heteropentamer combinations of seven subunit types; alpha, beta, gamma, δ, epsilon, theta, and π. Three subunits, alpha, beta, and gamma, have at least three separate gene products in mammals, and typical GABAA receptors have some combination of alpha, beta and gamma subunits. The rat beta 2 isoform is a 55 kDa, 450 amino acid (aa), 4 transmembrane protein with two terminal extracellular regions. The ligand-binding region is in the N-terminus (aa 13 - 218). The beta 2 subunit is part of the most common GABAA receptor combination in the mammalian brain ( alpha 1 beta 2 gamma 2). Like the other two beta subunits, beta 2 is phosphorylated on a consensus phosphorylation site (S410 of the precursor) that exists in the second cytoplasmic domain. In contrast to the other beta sub-units, only PKC is capable of this modification. However, tyrosine phosphorylation of beta 2 is known, and this apparently increases GABAA current amplitude.
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McDonald, B.J. et al. (1998) Nat. Neurosci. 1:23.
Wan, Q. et al. (1997) J. Neurosci. 17:5062.
gamma-Aminobutyric Acid Type A Receptor, beta-2 Polypeptide
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