Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, Immunoprecipitation (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all GAK Primary Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 675 to 725 of human Cyclin G Associated Kinase using the numbering given in entry NP_005246.2 (GeneID 2580).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GAK Antibody - BSA Free

Western Blot: GAK Antibody [NBP2-32181]

Western Blot: GAK Antibody [NBP2-32181]

Western Blot: GAK Antibody [NBP2-32181] - Whole cell lysate (10 ug) from CT26, CH27, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GAK antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Western Blot: GAK Antibody [NBP2-32181]

Western Blot: GAK Antibody [NBP2-32181]

Western Blot: GAK Antibody [NBP2-32181] - Whole cell lysate (10 ug) from GaMG, HeLa, HEK293T, Jurkat, andRKO cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GAK antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for GAK Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:2000 - 1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: GAK

In all eukaryotes, the cell cycle is governed by cyclin-dependent protein kinases (CDKs), whose activities are regulated by cyclins and CDK inhibitors in a diverse array of mechanisms that involve the control of phosphorylation and dephosphorylation of Ser, Thr or Tyr residues. Cyclins are molecules that possess a consensus domain called the 'cyclin box.' In mammalian cells, 9 cyclin species have been identified, and they are referred to as cyclins A through I. Cyclin G is a direct transcriptional target of the p53 tumor suppressor gene product and thus functions downstream of p53. GAK is an association partner of cyclin G and CDK5.

Long Name

Cyclin G-associated Kinase

Alternate Names

Auxillin 2

Gene Symbol

GAK

UniProt

NP_005246.2 (Human)

Additional GAK Products

Product Documents for GAK Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for GAK Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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