CRF21 (Cytochrome c-releasing factor 21; also C7orf24 or LOC79017) is a 21 kDa AIG2-like family member. It is a cytosolic protein that induces the release of Cytochrome c from mitochondria, possibly initiating apoptosis. Human CRF21 is 188 amino acids (aa) in length and is a complex of beta-sheets and alpha-helices that create two terminal domains. It has been proposed that CRF21 forms homodimers. There are at least two possible splice variants of human CRF21, one showing a 70 aa substitution, while the second variant shows an 18 aa substitution for aa 97-188. Full-length human CRF21 is 82% aa identical to mouse CRF21.
gamma-Glutamylcyclotransferase/CRF21/GGCT Antibody - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-12540
![Western Blot: gamma-Glutamylcyclotransferase/CRF21/GGCT Antibody [NBP3-12540]](https://resources.rndsystems.com/images/products/gamma-Glutamylcyclotransferase-CRF21-GGCT-Antibody-Western-Blot-NBP3-12540-img0005.jpg "Western Blot: gamma-Glutamylcyclotransferase/CRF21/GGCT Antibody [NBP3-12540]")
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Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
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Product Specifications
Immunogen
Produced in rabbits immunized with purified, recombinant Human gamma-Glutamylcyclotransferase/CRF21/GGCT (Accession#: O75223-1; Met1-Leu188)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Scientific Data Images
Western Blot: gamma-Glutamylcyclotransferase/CRF21/GGCT Antibody [NBP3-12540]
Western Blot: gamma-Glutamylcyclotransferase/CRF21/GGCT Antibody [NBP3-12540] - Lane A: 293 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:21 kDaObserved band size:21 kDa(We are unsure as to the identity of these extra bands.)![Immunohistochemistry-Paraffin: gamma-Glutamylcyclotransferase/CRF21/GGCT Antibody [NBP3-12540]](https://resources.rndsystems.com/images/products/gamma-Glutamylcyclotransferase-CRF21-GGCT-Antibody-Immunohistochemistry-Paraffin-NBP3-12540-img0003.jpg "Immunohistochemistry-Paraffin: gamma-Glutamylcyclotransferase/CRF21/GGCT Antibody [NBP3-12540]")
Immunohistochemistry-Paraffin: gamma-Glutamylcyclotransferase/CRF21/GGCT Antibody [NBP3-12540]
Immunohistochemistry-Paraffin: gamma-Glutamylcyclotransferase/CRF21/GGCT Antibody [NBP3-12540] - Staining of human GGCT in human corpus uteri with rabbit polyclonal antibody (1:10000).![Immunohistochemistry-Paraffin: gamma-Glutamylcyclotransferase/CRF21/GGCT Antibody [NBP3-12540]](https://resources.rndsystems.com/images/products/gamma-Glutamylcyclotransferase-CRF21-GGCT-Antibody-Immunohistochemistry-Paraffin-NBP3-12540-img0002.jpg "Immunohistochemistry-Paraffin: gamma-Glutamylcyclotransferase/CRF21/GGCT Antibody [NBP3-12540]")
Immunohistochemistry-Paraffin: gamma-Glutamylcyclotransferase/CRF21/GGCT Antibody [NBP3-12540]
Immunohistochemistry-Paraffin: gamma-Glutamylcyclotransferase/CRF21/GGCT Antibody [NBP3-12540] - Staining of human GGCT in human hepatoma with rabbit polyclonal antibody (1:10000).![Immunoprecipitation: gamma-Glutamylcyclotransferase/CRF21/GGCT Antibody [NBP3-12540]](https://resources.rndsystems.com/images/products/gamma-Glutamylcyclotransferase-CRF21-GGCT-Antibody-Immunoprecipitation-NBP3-12540-img0004.jpg "Immunoprecipitation: gamma-Glutamylcyclotransferase/CRF21/GGCT Antibody [NBP3-12540]")
Immunoprecipitation: gamma-Glutamylcyclotransferase/CRF21/GGCT Antibody [NBP3-12540]
Immunoprecipitation: gamma-Glutamylcyclotransferase/CRF21/GGCT Antibody [NBP3-12540] - Lane A:0.5 mg 293T Whole Cell Lysate2 uL anti-GGCT rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-GGCT rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 21 kDaObserved band size: 21 kDa
Immunohistochemistry-Paraffin: gamma-Glutamylcyclotransferase/CRF21/gamma-Glutamylcyclotransferase/CRF21/GGCT Antibody [NBP3-12540] -
Immunohistochemistry-Paraffin: gamma-Glutamylcyclotransferase/CRF21/gamma-Glutamylcyclotransferase/CRF21/GGCT Antibody [NBP3-12540] - Immunochemical staining of human gamma-Glutamylcyclotransferase/CRF21/GGCT in human lung cancer with NBP3-12540 (1:10000, formalin-fixed paraffin embedded sections).Applications
Application
Recommended Usage
ELISA
1:5000-1:10000
Immunohistochemistry
1:2500-1:10000
Immunohistochemistry-Paraffin
1:2500-1:10000
Immunoprecipitation
1-4 uL/mg of lysate
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
0.2 um filtered solution in PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: gamma-Glutamylcyclotransferase/CRF21
Long Name
Cytochrome c Releasing Factor 21
Alternate Names
C7orf24, CRF21, gammaGlutamylcyclotransferase, GCTG, GGC, GGCT, LOC79017
Gene Symbol
GGCT
Additional gamma-Glutamylcyclotransferase/CRF21 Products
Product Documents
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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