gamma Sarcoglycan Overexpression Lysate

Name: gamma Sarcoglycan Overexpression Lysate
Brand: Novus Biologicals
SKU: NBP2-11158
Price: 689 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-11158

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Product Summary
Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species

Human

Applications

Western Blot

Product Summary for gamma Sarcoglycan Overexpression Lysate

gamma Sarcoglycan Transient Overexpression Lysate

Expression Host: HEK293T

Plasmid: RC220093

Accession#: NM_000231

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.

Product Specifications

Application Notes

This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.

Theoretical MW

32.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Type

Overexpression

Scientific Data Images for gamma Sarcoglycan Overexpression Lysate

Western Blot: gamma Sarcoglycan Overexpression Lysate [NBP2-11158]

Western Blot: gamma Sarcoglycan Overexpression Lysate (Adult Normal) [NBP2-11158] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for gamma Sarcoglycan.

Formulation, Preparation, and Storage

Formulation

RIPA buffer

Concentration

The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -80C. Avoid freeze-thaw cycles.

Background: gamma-Sarcoglycan/SGCG

gamma Sarcoglycan encodes gamma-sarcoglycan, one of several sarcolemmal transmembrane glycoproteins that interact with dystrophin. The dystrophin-glycoprotein complex (DGC) spans the sarcolemma and is comprised of dystrophin, syntrophin, alpha- and beta-dystroglycans and sarcoglycans. The DGC provides a structural link between the subsarcolemmal cytoskeleton and the extracellular matrix of muscle cells. Defects in the encoded protein can lead to early onset autosomal recessive muscular dystrophy, in particular limb-girdle muscular dystrophy, type 2C (LGMD2C). [provided by RefSeq]

Long Name

Sarcoglycan, Gamma (35kDa Dystrophin-Associated Glycoprotein)

Alternate Names

DAGA4, DMDA, DMDA1, LGMD2C, LGMDR5, MGC130048, SCARMD2, SCG3

Gene Symbol

SGCG

Additional gamma-Sarcoglycan/SGCG Products

Product Documents for gamma Sarcoglycan Overexpression Lysate

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Certificate of Analysis

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Product Specific Notices for gamma Sarcoglycan Overexpression Lysate

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Related Research Areas

Proteoglycans

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