Gasdermin D Antibody (PD00-18) - N-terminal

Novus Biologicals | Catalog # NBP3-32369

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # PD00-18 expressed in HEK293
View all Gasdermin D Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein within Gasdermin D full length protein. (Uniprot: P57764)

Localization

Cell membrane, Secreted.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Gasdermin D Antibody (PD00-18) - N-terminal

Gasdermin D Antibody (PD00-18) - N-terminal Western Blot: Gasdermin D Antibody (PD00-18) - N-terminal [NBP3-32369]

Western Blot: Gasdermin D Antibody (PD00-18) - N-terminal [NBP3-32369]

Western blot analysis on different lysates with NBP3-32369 at 1/2,000 dilution. Lane 1: SiHa cell lysate Lane 2: Jurkat cell lysate Lane 3: THP-1 cell lysate Lane 4: PC-3M cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: PC-12 cell lysate Lane 7: L6 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 53/30 kDa Observed band size: 53/30 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32369 at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Gasdermin D Antibody (PD00-18) - N-terminal Western Blot: Gasdermin D Antibody (PD00-18) - N-terminal [NBP3-32369]

Western Blot: Gasdermin D Antibody (PD00-18) - N-terminal [NBP3-32369]

Western blot analysis on different lysates with NBP3-32369 at 1/2,000 dilution. Lane 1: THP-1 cell lysate (20 µg/Lane) Lane 2: THP-1 treated with 100nM TPA overnight then add 100ng/mL LPS for 7 hours then add 1μg/mL BFA for 3 hours cell lysate (20 µg/Lane) Predicted band size: 53/30 kDa Observed band size: 53/35 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32369 at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Applications for Gasdermin D Antibody (PD00-18) - N-terminal

Application
Recommended Usage

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH7.4), 0.1% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Gasdermin D

Gasdermin D is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. Gasdermin D has been suggested to act as a tumor suppressor. Alternatively spliced transcript variants have been described. [provided by RefSeq, Oct 2009]

Alternate Names

DF5L, DFNA5L, gasdermin domain containing 1, Gasdermin domain-containing protein 1, gasdermin-D, GSDMDC1

Gene Symbol

GSDMD

Additional Gasdermin D Products

Product Documents for Gasdermin D Antibody (PD00-18) - N-terminal

Certificate of Analysis

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Product Specific Notices for Gasdermin D Antibody (PD00-18) - N-terminal

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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