Gastrin-releasing Peptide R/GRPR Overexpression Lysate

Novus Biologicals | Catalog # NBP2-04290

Novus Biologicals
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Key Product Details

Species

Human

Applications

Western Blot

Product Summary for Gastrin-releasing Peptide R/GRPR Overexpression Lysate

Gastrin-releasing Peptide R/GRPR Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC210362

Accession#: NM_005314

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.

Product Specifications

Application Notes

This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.

Theoretical MW

43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Type

Overexpression

Scientific Data Images for Gastrin-releasing Peptide R/GRPR Overexpression Lysate

Western Blot: Gastrin-releasing Peptide R/GRPR Overexpression Lysate [NBP2-04290]

Western Blot: Gastrin-releasing Peptide R/GRPR Overexpression Lysate [NBP2-04290]

Western Blot: GRPR Overexpression Lysate (Adult Normal) [NBP2-04290] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GRPR.

Formulation, Preparation, and Storage

Formulation

RIPA buffer

Concentration

The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -80C. Avoid freeze-thaw cycles.

Background: Gastrin-releasing Peptide R/GRPR

GRPR mediates signals of cellular proliferation and it has been shown to be aberrantly expressed in several cancers such as colon, lung, ovary, pancreas and prostate. It also expressed at very high levels in normal pancreas and at lower levels in brain, breast, colon, lung, lymph node, ovary, placenta, prostate, stomach and uterus.

Long Name

Gastrin-releasing Peptide Receptor

Alternate Names

Gastrin-releasing PeptideR, Gastrinreleasing PeptideR, GRPR

Gene Symbol

GRPR

Additional Gastrin-releasing Peptide R/GRPR Products

Product Documents for Gastrin-releasing Peptide R/GRPR Overexpression Lysate

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Gastrin-releasing Peptide R/GRPR Overexpression Lysate

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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