GFP Alexa Fluor® 647-conjugated Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
FAB42402R-100UG
R&D Systems Antibodies
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GFP Alexa Fluor® 647-conjugated Antibody Summary

Specificity
Detects recombinant GFPuv and eGFP in Western blots.
Source
Monoclonal Mouse IgG1 Clone # 454505R
Immunogen
E.coli-derived recombinant GFPuv Ser2-Lys238 Accession # P42212
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 647

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
HEK293 human embryonic kidney cell line transfected with eGFP

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

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Preparation and Storage

Stability & Storage
Store the unopened product at 2 - 8 degreesC. Do not use past expiration date.

Background: GFP

Green fluorescent protein (GFP) is a 27 kDa protein originally isolated from the jellyfish Aequorea victoria. In the presence of UV light (490-520 nm), it emits a green fluorescent color that can be used to pinpoint locations of various intracellular proteins. GFP is 238 amino acids (aa) in length. It is a globular monomer that has a tendency to dimerize. The monomer has the shape of a beta -barrel with a chromophore (aa 65-67) containing alpha -helix running up its center. GFPuv is the Aequorea sequence with three aa substitutions; Phe to Ser at # 99, Met to Thr at # 153, and Val to Ala at # 163. This form expresses faster and is 18-fold brighter than native GFP; excitation peaks at 395 nm and emission at 508 nm.

Long Name
Green Fluorescent Protein
Alternate Names
eGFP; Enhanced Green Fluorescent Protein; GFP; GFPuv; green fluorescent protein (gfp); Green Fluorescent Protein

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