Key Product Details

Species Reactivity

Multi-Species

Applications

Flow Cytometry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 Clone # 454505R
View all GFP Primary Antibodies »

Product Specifications

Immunogen

E.coli-derived recombinant GFPuv
Ser2-Lys238
Accession # P42212

Specificity

Stains eGFP transfectants but not irrelevant transfectants in flow cytometry. Detects recombinant GFPuv in Western blots. For optimal Western blot results, we recommend R&D Systems Goat Anti-GFP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4240) or Novus Biologicals Mouse Anti-GFP Monoclonal Antibody (Catalog # NBP2-2211).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for GFP Antibody

Detection of eGFP antibody in HEK293 Human Cell Line Transfected with eGFP antibody by Flow Cytometry.

Detection of eGFP in HEK293 Human Cell Line Transfected with eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with eGFP was stained with either (A) Mouse Anti-GFP Monoclonal Antibody (Catalog # MAB42401) or (B) Mouse IgG1 Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).
GFP antibody in HEK293 Human Cell Line by Immunocytochemistry (ICC).

GFP in HEK293 Human Cell Line.

GFP was detected in immersion fixed HEK293 human embryonic kidney cell line transfected with GFP (green, upper panel) using Mouse Anti-GFP Monoclonal Antibody (Catalog # MAB42401R) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, lower panel; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm in GFP-positive cells. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for GFP Antibody

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: HEK293 human embryonic kidney cell line transfected with eGFP

Immunocytochemistry

5-25 µg/mL
Sample: Immersion fixed HEK293 human embryonic kidney cell line transfected with GFP

Reviewed Applications

Read 1 review rated 5 using MAB42401R in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GFP

Green fluorescent protein (GFP) is a 27 kDa protein originally isolated from the jellyfish Aequorea victoria. In the presence of UV light (490-520 nm), it emits a green fluorescent color that can be used to pinpoint locations of various intracellular proteins. GFP is 238 amino acids (aa) in length. It is a globular monomer that has a tendency to dimerize. The monomer has the shape of a beta -barrel with a chromophore (aa 65-67) containing alpha -helix running up its center. GFPuv is the Aequorea sequence with three aa substitutions; Phe to Ser at # 99, Met to Thr at # 153, and Val to Ala at # 163. This form expresses faster and is 18-fold brighter than native GFP; excitation peaks at 395 nm and emission at 508 nm.

Long Name

Green Fluorescent Protein

Alternate Names

eGFP, GFPuv

UniProt

P42212

Additional GFP Products

Product Documents for GFP Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for GFP Antibody

For research use only

Customer Reviews for GFP Antibody (1)

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  • GFP Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Liver tissue
    Species: Mouse
    Verified Customer | Posted 10/30/2017

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Protocols

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