GIF Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-81610

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all GIF Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LFGQVLKDIVEKISMKIKDNGIIGDIYSTGLAMQALSVTPEPSKKEWNCKKTTDMILNEIKQGKFHNPMSIAQILPSLKGKTYLDVPQVTCSPDHEVQPTLPSNP

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (80%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GIF Antibody - BSA Free

Immunohistochemistry-Paraffin: GIF Antibody [NBP1-81610]

Immunohistochemistry-Paraffin: GIF Antibody [NBP1-81610]

Immunohistochemistry-Paraffin: GIF Antibody [NBP1-81610] - Analysis in human stomach and skin tissues. Corresponding GIF RNA-seq data are presented for the same tissues.
Western Blot: GIF Antibody [NBP1-81610]

Western Blot: GIF Antibody [NBP1-81610]

Western Blot: GIF Antibody [NBP1-81610] - Analysis in control (vector only transfected HEK293T lysate) and GIF over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: GIF Antibody [NBP1-81610]

Immunohistochemistry-Paraffin: GIF Antibody [NBP1-81610]

Immunohistochemistry-Paraffin: GIF Antibody [NBP1-81610] - Staining of human stomach shows strong cytoplasmic positivity in Parietal cells.
Immunohistochemistry-Paraffin: GIF Antibody [NBP1-81610]

Immunohistochemistry-Paraffin: GIF Antibody [NBP1-81610]

Immunohistochemistry-Paraffin: GIF Antibody [NBP1-81610] - Staining of human skin shows no positivity in squamous epithelial cells as expected.
Immunohistochemistry-Paraffin: GIF Antibody [NBP1-81610]

Immunohistochemistry-Paraffin: GIF Antibody [NBP1-81610]

Immunohistochemistry-Paraffin: GIF Antibody [NBP1-81610] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: GIF Antibody [NBP1-81610]

Immunohistochemistry-Paraffin: GIF Antibody [NBP1-81610]

Immunohistochemistry-Paraffin: GIF Antibody [NBP1-81610] - Staining of human kidney shows no positivity in cells in tubules as expected.

Applications for GIF Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Reviewed Applications

Read 1 review rated 5 using NBP1-81610 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GIF

GIF is a member of the cobalamin transport protein family. It encodes a glycoprotein secreted by parietal cells of the gastric mucosa and is required for adequate absorption of vitamin B12. Vitamin B12 is necessary for erythrocyte maturation and mutations in this gene may lead to congenital pernicious anemia. [provided by RefSeq]

Long Name

Gastric Intrinsic Factor

Alternate Names

IF, IFMH, INF, TCN3

Gene Symbol

CBLIF

Additional GIF Products

Product Documents for GIF Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for GIF Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for GIF Antibody - BSA Free

Customer Reviews for GIF Antibody - BSA Free (1)

5 out of 5
1 Customer Rating
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used GIF Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • GIF Antibody
    Name: Feng Li
    Application: Immunocytochemistry
    Sample Tested: Stomach tissue
    Species: mouse stomach and Mouse
    Verified Customer | Posted 05/16/2017
    Immunofluorescence staining for stomach section of normal wild type C57 mouse. Dilution is 1:100. Very good and stable antibody.
    GIF Antibody - BSA Free NBP1-81610

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies