GINS4 Antibody - BSA Free

Western Blot: GINS4 Antibody - BSA Free [NBP2-16659] -

miR-494 regulates target genes in cell survival and DNA replication. (A) Venn diagram showing the number of downregulated target proteins in a Tandem Mass Tag labeled Mass Spectrometry profile from HUVECs treated with TCN or transfected with miR-494 compared to vehicle treatment or control miR respectively. (B) Fold-change (compared to respective controls) of protein or mRNA levels as assessed by Mass Spectrometry or qRT-PCR respectively for the six targets that were downregulated in both TCN and miR-494 groups. All six targets harbor miR-494 binding sites in their 3′UTRs. (C) Representative Simple Western blot showing survivin (BIRC5) and GINS4 levels in HUVECs 24 h after miR-494 transfection followed by TCN treatment (24 h). Right panels show quantitation of biological replicates. ∗P < 0.05, ∗∗P < 0.01, by two-tailed Student’s T-test. (D) Immunofluorescence images showing survivin expression in HUVECs 24 h after miR transfection and/or TCN treatment. Right panel shows quantification via ImageJ of survivin fluorescence intensity in each cell. Each dot represents individual cells. Scale bar in white = 50 μm. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001 by one-way ANOVA with post hoc Tukey’s correction. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34322482), licensed under a CC-BY license. Not internally tested by Novus Biologicals.