Girdin (GIRDers of actIN filaments; also APE and GIV) is a member of the CCDC88 family of molecules. It is a cytosolic protein, ubiquitously expressed, and plays a key role in the motility of breast cancer cells. Human Girdin is 1871 amino acids (aa) in length. It contains two coiled-coil regions that mediate homodimerization (aa 196-425 and 458-1358), and one phosphoinositide-binding domain (aa 1390-1408). Normally, Girdin is membrane-associated via an interaction with phosphoinositides. Upon phosphorylation at Ser1417 by Akt, it relocates to lamellipodia where it crosslinks actin filaments. Multiple isoforms of Girdin have been detected.
Girdin Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-71856
![Immunoprecipitation: Girdin Antibody [NBP1-71856]](https://resources.rndsystems.com/images/products/Girdin-Antibody-Western-Blot-NBP1-71856-img0002.jpg "Immunoprecipitation: Girdin Antibody [NBP1-71856]")
Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Human
Applications
Immunoprecipitation, Western Blot (Negative)
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
The immunogen for this product maps to a region between residue 425 and 475 of human Coiled-Coil Domain Containing 88A using the numbering given in entry NP_001129069.1 (GeneID 55704).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Girdin Antibody - BSA Free
Immunoprecipitation: Girdin Antibody [NBP1-71856]
Immunoprecipitation: Girdin Antibody [NBP1-71856] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-CCDC88A antibody used for IP at 6 mcg/mg lysate. CCDC88A was also immunoprecipitated by rabbit anti-CCDC88A antibody NBP1-71857, which recognizes a downstream epitope. For blotting immunoprecipitated CCDC88A, NBP1-71857 was used at 1 ug/ml.Applications for Girdin Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
2 - 10 ug/mg lysate
Application Notes
Western Blot Not recommended
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Girdin
Long Name
Girders of Actin Filament
Alternate Names
APE, CCDC88A, GIV, GRDN, HkRP1
Gene Symbol
CCDC88A
UniProt
Additional Girdin Products
Product Documents for Girdin Antibody - BSA Free
Certificate of Analysis
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Product Specific Notices for Girdin Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
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