GIT1 Antibody

Novus Biologicals | Catalog # NBP1-19141

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all GIT1 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 375 and 425 of human G protein-coupled receptor kinase interacting ArfGAP 1 using the numbering given in entry NP_054749.2 (GeneID 28964).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GIT1 Antibody

Western Blot: GIT1 Antibody [NBP1-19141]

Western Blot: GIT1 Antibody [NBP1-19141]

Western Blot: GIT1 Antibody [NBP1-19141] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. NBP1-19141 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. GIT1 was also immunoprecipitated by NBP1-19142, which recognizes a downstream epitope.
Immunohistochemistry-Paraffin: GIT1 Antibody [NBP1-19141]

Immunohistochemistry-Paraffin: GIT1 Antibody [NBP1-19141]

Immunohistochemistry-Paraffin: GIT1 Antibody [NBP1-19141] - Human breast carcinoma. Antibody used at a dilution of 1:200.
Immunohistochemistry-Paraffin: GIT1 Antibody [NBP1-19141]

Immunohistochemistry-Paraffin: GIT1 Antibody [NBP1-19141]

Immunohistochemistry-Paraffin: GIT1 Antibody [NBP1-19141] - FFPE section of human breast carcinoma. Affinity purified rabbit anti-GIT1 used at a dilution of 1:250.

Applications for GIT1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: GIT1

G-protein-coupled receptor kinase interactor 1 (GIT1) and G-protein-coupled receptor kinase interactor 2 (GIT2) are GTPase-activating proteins for ADP-ribosylation (Arf) GTPases. GIT1 and GIT2 regulate Arf activity and the sequestration of activated G protein-coupled receptors from the surface of the cell. These activities have effects on multiple cellular processes such as cell migration, T-cell activation, and centrosome dynamics. Both GIT proteins also serve as scaffolds to link signaling molecules. Alternative names for GIT1 include GRK-interacting protein 1, cool-associated and tyrosine phosphorylated protein 1, and Cat-1.

Long Name

G Protein-Coupled Receptor Kinase Interacting ArfGAP 1

Alternate Names

ARF GAP GIT1

Entrez Gene IDs

28964 (Human)

Gene Symbol

GIT1

UniProt

NP_054749.2 (Human)

Additional GIT1 Products

Product Documents for GIT1 Antibody

Certificate of Analysis

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Product Specific Notices for GIT1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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