GKAP/DLGAP1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-13181

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Predicted:

Bovine (100%), Human (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all GKAP/DLGAP1 Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of mouse GKAP/DLGAP1. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Centrifuge briefly prior to opening.

Scientific Data Images for GKAP/DLGAP1 Antibody - BSA Free

Western Blot: GKAP/DLGAP1 Antibody [NBP3-13181]

Western Blot: GKAP/DLGAP1 Antibody [NBP3-13181]

Western Blot: GKAP/DLGAP1 Antibody [NBP3-13181] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GKAP/DLGAP1 antibody (NBP3-13181) diluted at 1:3000.
Immunocytochemistry/ Immunofluorescence: GKAP/DLGAP1 Antibody [NBP3-13181]

Immunocytochemistry/ Immunofluorescence: GKAP/DLGAP1 Antibody [NBP3-13181]

Immunocytochemistry/Immunofluorescence: GKAP/DLGAP1 Antibody [NBP3-13181] - GKAP/DLGAP1 antibody detects GKAP/DLGAP1 protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GKAP/DLGAP1 stained by GKAP/DLGAP1 antibody (NBP3-13181) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (NBP3-13505) diluted at 1:500. Blue: Fluoroshield with DAPI.
Immunohistochemistry-Frozen: GKAP/DLGAP1 Antibody [NBP3-13181]

Immunohistochemistry-Frozen: GKAP/DLGAP1 Antibody [NBP3-13181]

Immunohistochemistry-Frozen: GKAP/DLGAP1 Antibody [NBP3-13181] - GKAP/DLGAP1 antibody detects GKAP/DLGAP1 Protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse cerebellum. Green: GKAP/DLGAP1 stained by GKAP/DLGAP1 antibody (NBP3-13181) diluted at 1:250. Red: NF-H, stained by NF-H antibody [GT114] diluted at 1:500. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 10 min
Western Blot: GKAP/DLGAP1 Antibody [NBP3-13181]

Western Blot: GKAP/DLGAP1 Antibody [NBP3-13181]

Western Blot: GKAP/DLGAP1 Antibody [NBP3-13181] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GKAP/DLGAP1 antibody (NBP3-13181) diluted at 1:3000.
Immunohistochemistry-Paraffin: GKAP/DLGAP1 Antibody [NBP3-13181]

Immunohistochemistry-Paraffin: GKAP/DLGAP1 Antibody [NBP3-13181]

Immunohistochemistry-Paraffin: GKAP/DLGAP1 Antibody [NBP3-13181] - GKAP/DLGAP1 antibody detects GKAP/DLGAP1 protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. GKAP/DLGAP1 antibody (NBP3-13181) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: GKAP/DLGAP1 Antibody [NBP3-13181]

Immunohistochemistry-Paraffin: GKAP/DLGAP1 Antibody [NBP3-13181]

Immunohistochemistry-Paraffin: GKAP/DLGAP1 Antibody [NBP3-13181] - GKAP/DLGAP1 antibody detects GKAP/DLGAP1 protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. GKAP/DLGAP1 antibody (NBP3-13181) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications for GKAP/DLGAP1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GKAP/DLGAP1

Members of the postsynaptic density-95 (PSD-95)/SAP90 family of membraneassociated guanylate kinase (MAGUK) proteins function as multimodular scaffolds that organize protein-signaling complexes at neuronal synapses. PSD-95/SAP90 binds guanylate kinase-associated protein (GKAP), also designated GK domain-binding protein, DAP-1-alpha, DAP-1-beta, PSD-95 binding protein, PSD-95/SAP90 associated protein, or SAPAP1, through the guanylate kinase domain. SAPAP1 is expressed widely in neurons of the cortex and hippocampus and in the Purkinje and granule cells of the cerebellum. N-terminal splice variants of SAPAP1 are expressed as 95 and 130 kDa proteins. SAPAP1 is localized specifically in the PSD of glutamatergic synapses, consistent with its direct interaction with PSD-95 family proteins.

Long Name

Guanylate Kinase-associated Protein

Alternate Names

DAP1, DLGAP1, SAPAP1

Gene Symbol

DLGAP1

Additional GKAP/DLGAP1 Products

Product Documents for GKAP/DLGAP1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for GKAP/DLGAP1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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