Rat GLDC ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP3-53167

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Tissue homogenates, cell lysates and other biological fluids (100 uL)

Sensitivity

0.053 ng/mL (example only; lot dependent)

Assay Range

0.156 - 10 ng/mL (example only; lot dependent)
View all GLDC ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Rat

Description

Assay Length: 4.5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV 10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV 12 (example only; lot dependent)

Kit Contents for Rat GLDC ELISA Kit (Colorimetric)

  • Pre-coated 96T strip plate, Plate sealer for 96 wells, Standard, Diluent Buffer, Detection Reagent A, Detection Reagent B, TMB Substrate, Stop Solution, Wash Buffer (30 x concentrate), Instruction manual

Preparation and Storage

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: GLDC

The enzyme system for cleavage of glycine (glycine cleavage system; GCS; EC 2.1.2.10), which is confined to themitochondria, is composed of 4 protein components: P protein (a pyridoxal phosphate-dependent glycine decarboxylase),H protein (a lipoic acid-containing protein), T protein (a tetrahydrofolate-requiring enzyme), and L protein (alipoamide dehydrogenase). Glycine encephalopathy (GCE; MIM 605899) may be due to a defect in any one of these enzymes;see MIM 238310, MIM 238330, and MIM 238331.(supplied by OMIM)

Alternate Names

EC 1.4.4.2, GCE, GCSPMGC138198, Glycine cleavage system P protein, glycine cleavage system protein P, Glycine decarboxylase, glycine decarboxylase P-protein, glycine dehydrogenase (decarboxylating), glycine dehydrogenase (decarboxylating; glycine decarboxylase, glycine cleavagesystem protein P), glycine dehydrogenase [decarboxylating], mitochondrial, HYGN1, MGC138200, NKH

Gene Symbol

GLDC

Additional GLDC Products

Product Documents for Rat GLDC ELISA Kit (Colorimetric)

Certificate of Analysis

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Product Specific Notices for Rat GLDC ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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