GLI-3 Antibody (8B1E2)

Novus Biologicals | Catalog # NBP3-16166

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 8B1E2 expressed in HEK293
View all GLI-3 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1301-1580 of human GLI-3 (P10071). PNESAGSMVNGMQNQDPVGQGYLAHQLLGDSMQHPGAGRPGQQMLGQISATSHINIYQGPESCLPGAHGMGSQPSSLAVVRGYQPCASFGGSRRQAMPRDSLALQSGQLSDTSQTCRVNGIKMEMKGQPHPLCSNLQNYSGQFYDQTVGFSQQDTKAGSFSISDASCLLQGTSAKNSELLSPGANQVTSTVDSLDSHDLEGVQIDFDAIIDDGDHSSLMSGALSPSIIQNLSHSSSRLTTPRASLPFPALSMSTTNMAIGDMSSLLTSLAEESKFLAVMQ

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

170 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GLI-3 Antibody (8B1E2)

Western Blot: GLI-3 Antibody (8B1E2) [NBP3-16166]

Western Blot: GLI-3 Antibody (8B1E2) [NBP3-16166]

Western Blot: GLI-3 Antibody (8B1E2) [NBP3-16166] - Western blot analysis of extracts of HeLa cells, using GLI-3 antibody (NBP3-16166) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
GLI-3 Antibody (8B1E2)

Immunocytochemistry/ Immunofluorescence: GLI-3 Antibody (8B1E2) [NBP3-16166] -

Immunocytochemistry/ Immunofluorescence: GLI-3 Antibody (8B1E2) [NBP3-16166] - Immunofluorescence analysis of NIH/3T3 cells using GLI-3 Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
GLI-3 Antibody (8B1E2)

Immunocytochemistry/ Immunofluorescence: GLI-3 Antibody (8B1E2) [NBP3-16166] -

Immunocytochemistry/ Immunofluorescence: GLI-3 Antibody (8B1E2) [NBP3-16166] - Immunofluorescence analysis of U-2 OS cells using GLI-3 Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
GLI-3 Antibody (8B1E2)

Immunocytochemistry/ Immunofluorescence: GLI-3 Antibody (8B1E2) [NBP3-16166] -

Immunocytochemistry/ Immunofluorescence: GLI-3 Antibody (8B1E2) [NBP3-16166] - Immunofluorescence analysis of C6 cells using GLI-3 Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Applications for GLI-3 Antibody (8B1E2)

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 ug/mL

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GLI-3

Gli-3 (also known as Zinc Finger Protein Gli-3 or GLI-Kruppel family member GLI-3) belongs to the GLI C2H2-type zinc-finger protein family and contains 5 C2H2-type zinc fingers. Gli-3 is very important for normal limb and brain development and is implicated in the transduction of Shh signal. Gli-3 is a nuclear protein expressed in a wide variety of normal adult tissues, including lung, colon, spleen, placenta, testis, and myometrium. Defects in Gli-3 are the cause of Greig cephalo-polysyndactyly syndrome (GCPS); an autosomal dominant disorder-affecting limb and craniofacial development. Two isoforms of human Gli-3 have been reported. One is the fulllength protein at ~170-190kDa and the other is a truncated isoform at ~80kDa.

Long Name

GLI-Kruppel family member GLI3

Alternate Names

ACLS, ADD, Bph, GCPS, GLI3, PAPA1, PAPB, Pdn, PHS, PPDIV, Xt

Gene Symbol

GLI3

Additional GLI-3 Products

Product Documents for GLI-3 Antibody (8B1E2)

Certificate of Analysis

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Product Specific Notices for GLI-3 Antibody (8B1E2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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Associated Pathways

TGF-beta Signaling Pathways TGF-beta Signaling Pathway Thumbnail