GLI-3 Antibody

Novus Biologicals | Catalog # NBP2-16665

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Chicken (92%), Chimpanzee (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
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Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human GLI-3. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (87%).

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

170 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GLI-3 Antibody

Immunocytochemistry/ Immunofluorescence: GLI-3 Antibody [NBP2-16665]

Immunocytochemistry/ Immunofluorescence: GLI-3 Antibody [NBP2-16665]

Immunocytochemistry/Immunofluorescence: GLI-3 Antibody [NBP2-16665] - Analysis of paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: GLI-3 Antibody [NBP2-16665]

Immunohistochemistry-Paraffin: GLI-3 Antibody [NBP2-16665]

Immunohistochemistry-Paraffin: GLI-3 Antibody [NBP2-16665] - Human lung adenocarcinoma. GLI3 antibody diluted at 1:250. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
GLI-3 Antibody

Western Blot: GLI-3 Antibody [NBP2-16665] -

Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with GLI3 antibody (NBP2-16665) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Applications for GLI-3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: GLI-3

This gene encodes a protein which belongs to the C2H2-type zinc finger proteins subclass of the Gli family. They are characterized as DNA-binding transcription factors and are mediators of Sonic hedgehog (Shh) signaling. The protein encoded by this gene localizes in the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. It is also thought to play a role during embryogenesis. Mutations in this gene have been associated with several diseases, including Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, and postaxial polydactyly types A1 and B. [provided by RefSeq]

Long Name

GLI-Kruppel family member GLI3

Alternate Names

ACLS, ADD, Bph, GCPS, GLI3, PAPA1, PAPB, Pdn, PHS, PPDIV, Xt

Gene Symbol

GLI3

UniProt

P10071

Additional GLI-3 Products

Product Documents for GLI-3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for GLI-3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

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Associated Pathways

TGF-beta Signaling Pathways TGF-beta Signaling Pathway Thumbnail