Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Immunoprecipitation, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # PCRP-GLIS3-1B11

Format

Azide and BSA Free
View all GLIS3 Primary Antibodies »

Product Specifications

Immunogen

Recombinant full-length human GLIS3 protein (Uniprot: Q8NEA6)

Localization

Nucleus

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

84 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-13869).

Antibody with azide store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for GLIS3 Antibody (PCRP-GLIS3-1B11) - Azide and BSA Free

Protein Array: GLIS3 Antibody (PCRP-GLIS3-1B11) - Azide and BSA Free [NBP3-14052]

Protein Array: GLIS3 Antibody (PCRP-GLIS3-1B11) - Azide and BSA Free [NBP3-14052]

Protein Array: GLIS3 Antibody (PCRP-GLIS3-1B11) - Azide and BSA Free [NBP3-14052] - Analysis of Protein Array containing more than 19,000 full-length human proteins using GLIS3 Antibody (PCRP-GLIS3-1B11).
GLIS3 Antibody (PCRP-GLIS3-1B11) - Azide and BSA Free Flow Cytometry: GLIS3 Antibody (PCRP-GLIS3-1B11) - Azide and BSA Free [NBP3-14052] -

Flow Cytometry: GLIS3 Antibody (PCRP-GLIS3-1B11) - Azide and BSA Free [NBP3-14052] -

Flow cytometric analysis of PFA-fixed HeLa cells. GLIS3 Antibody (PCRP-GLIS3-1B11) - Azide and BSA Free followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Applications for GLIS3 Antibody (PCRP-GLIS3-1B11) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunoprecipitation

1-2 ug per 100-500 ug protein
Application Notes
ELISA: For coating, order this antibody without BSA

Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: GLIS3

The GLIS3 gene is a member of the GLI-similar zinc finger protein family and encodes a nuclear protein with five C2H2-typezinc finger domains. This protein functions as both a repressor and activator of transcription and is specificallyinvolved in the developm

Alternate Names

FLJ38999, FLJ90578, GLIS family zinc finger 3, GLI-similar 3, OK/KNS-cl.4, zinc finger protein 515, zinc finger protein GLIS3

Gene Symbol

GLIS3

Additional GLIS3 Products

Product Documents for GLIS3 Antibody (PCRP-GLIS3-1B11) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for GLIS3 Antibody (PCRP-GLIS3-1B11) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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