Glucose 6 phosphate isomerase Antibody (12A4) - BSA Free

Novus Biologicals | Catalog # NBP3-26548

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 12A4 expressed in HEK293

Format

BSA Free
View all Glucose 6 phosphate isomerase Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from Human Glucose 6 phosphate isomerase [UniProt P06744]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Glucose 6 phosphate isomerase Antibody (12A4) - BSA Free

Glucose 6 phosphate isomerase Antibody (12A4)

Immunohistochemistry: Glucose 6 phosphate isomerase Antibody (12A4) [NBP3-26548] -

Immunohistochemistry: Glucose 6 phosphate isomerase Antibody (12A4) [NBP3-26548] - Image of Glucose 6 phosphate isomerase Antibody (12A4) diluted at 1:50 and staining in paraffin-embedded human prostate tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.45% DAB.
Glucose 6 phosphate isomerase Antibody (12A4)

Immunohistochemistry: Glucose 6 phosphate isomerase Antibody (12A4) [NBP3-26548] -

Immunohistochemistry: Glucose 6 phosphate isomerase Antibody (12A4) [NBP3-26548] - Image of Glucose 6 phosphate isomerase Antibody (12A4) diluted at 1:50 and staining in paraffin-embedded human prostate tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.45% DAB.
Glucose 6 phosphate isomerase Antibody (12A4)

Western Blot: Glucose 6 phosphate isomerase Antibody (12A4) [NBP3-26548] -

Western Blot: Glucose 6 phosphate isomerase Antibody (12A4) [NBP3-26548] - Positive Western Blot detected in: A549 whole cell lysate, U251 whole cell lysate, MCF7 whole cell lysate, HELA whole cell lysate, PC-3 whole cell lysate.
All lanes: Glucose 6 phosphate isomerase Antibody at 1: 1000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 63 kDa
Observed band size: 63 kDa
Glucose 6 phosphate isomerase Antibody (12A4)

Flow Cytometry: Glucose 6 phosphate isomerase Antibody (12A4) [NBP3-26548] -

Flow Cytometry: Glucose 6 phosphate isomerase Antibody (12A4) [NBP3-26548] - Overlay Peak curve showing A549 cells stained with Glucose 6 phosphate isomerase Antibody (12A4) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

Applications for Glucose 6 phosphate isomerase Antibody (12A4) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:50-1:200

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:2000

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: Glucose 6 phosphate isomerase

Glucose 6 phosphate isomerase belongs to the GPI family whose members encode multifunctional phosphoglucose isomerase proteins involved in energy pathways. The protein encoded by this gene is a dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. The protein functions in different capacities inside and outside the cell. In the cytoplasm, the gene product is involved in glycolysis and gluconeogenesis, while outside the cell it functions as a neurotrophic factor for spinal and sensory neurons. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment.

Alternate Names

AMFGNPI, Autocrine motility factor, DKFZp686C13233, EC 5.3.1.9, glucose phosphate isomerase, glucose-6-phosphate isomerase, hexose monophosphate isomerase, hexosephosphate isomerase, Neuroleukin, NLKSA36, oxoisomerase, PGI, PHI, Phosphoglucose isomerase, phosphohexomutase, Phosphohexose isomerase, phosphosaccharomutase, SA-36, Sperm antigen 36, sperm antigen-36

Gene Symbol

GPI

Additional Glucose 6 phosphate isomerase Products

Product Documents for Glucose 6 phosphate isomerase Antibody (12A4) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Glucose 6 phosphate isomerase Antibody (12A4) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Glucose 6 phosphate isomerase Antibody (12A4) - BSA Free

There are currently no reviews for this product. Be the first to review Glucose 6 phosphate isomerase Antibody (12A4) - BSA Free and earn rewards!

Have you used Glucose 6 phosphate isomerase Antibody (12A4) - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies