Glucose 6 phosphate isomerase Antibody (OTI2D2)

Novus Biologicals | Catalog # NBP2-02541

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Key Product Details

Species Reactivity

Human, Mouse, Rat, Monkey, Primate

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2D2
View all Glucose 6 phosphate isomerase Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human GPI(NP_000166) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

63 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Glucose 6 phosphate isomerase Antibody (OTI2D2)

Western Blot: Glucose 6 phosphate isomerase Antibody (2D2) [NBP2-02541] Analysis of extracts (35ug) from 9 different cell lines by using anti-Glucose 6 phosphate isomerasemonoclonal antibody.
Immunocytochemistry/ Immunofluorescence: Glucose 6 phosphate isomerase Antibody (OTI2D2) [NBP2-02541]

Immunocytochemistry/ Immunofluorescence: Glucose 6 phosphate isomerase Antibody (OTI2D2) [NBP2-02541]

Immunocytochemistry/Immunofluorescence: Glucose 6 phosphate isomerase Antibody (2D2) [NBP2-02541] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Glucose 6 phosphate isomerase.
Immunohistochemistry-Paraffin: Glucose 6 phosphate isomerase Antibody (OTI2D2) [NBP2-02541]

Immunohistochemistry-Paraffin: Glucose 6 phosphate isomerase Antibody (OTI2D2) [NBP2-02541]

Immunohistochemistry-Paraffin: Glucose 6 phosphate isomerase Antibody (2D2) [NBP2-02541] - Staining of paraffin-embedded Human prostate tissue using anti-Glucose 6 phosphate isomerasemouse monoclonal antibody.
Western Blot: Glucose 6 phosphate isomerase Antibody (OTI2D2) [NBP2-02541]

Western Blot: Glucose 6 phosphate isomerase Antibody (OTI2D2) [NBP2-02541]

Western Blot: Glucose 6 phosphate isomerase Antibody (2D2) [NBP2-02541] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Glucose 6 phosphate isomerase(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Glucose 6 phosphate isomerase.
Immunohistochemistry-Paraffin: Glucose 6 phosphate isomerase Antibody (OTI2D2) [NBP2-02541]

Immunohistochemistry-Paraffin: Glucose 6 phosphate isomerase Antibody (OTI2D2) [NBP2-02541]

Immunohistochemistry-Paraffin: Glucose 6 phosphate isomerase Antibody (2D2) [NBP2-02541] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Glucose 6 phosphate isomerasemouse monoclonal antibody.
Immunohistochemistry-Paraffin: Glucose 6 phosphate isomerase Antibody (OTI2D2) [NBP2-02541]

Immunohistochemistry-Paraffin: Glucose 6 phosphate isomerase Antibody (OTI2D2) [NBP2-02541]

Immunohistochemistry-Paraffin: Glucose 6 phosphate isomerase Antibody (2D2) [NBP2-02541] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-Glucose 6 phosphate isomerasemouse monoclonal antibody.
Immunohistochemistry-Paraffin: Glucose 6 phosphate isomerase Antibody (OTI2D2) [NBP2-02541]

Immunohistochemistry-Paraffin: Glucose 6 phosphate isomerase Antibody (OTI2D2) [NBP2-02541]

Immunohistochemistry-Paraffin: Glucose 6 phosphate isomerase Antibody (2D2) [NBP2-02541] - Staining of paraffin-embedded Human liver tissue using anti-Glucose 6 phosphate isomerasemouse monoclonal antibody.
Immunohistochemistry-Paraffin: Glucose 6 phosphate isomerase Antibody (OTI2D2) [NBP2-02541]

Immunohistochemistry-Paraffin: Glucose 6 phosphate isomerase Antibody (OTI2D2) [NBP2-02541]

Immunohistochemistry-Paraffin: Glucose 6 phosphate isomerase Antibody (2D2) [NBP2-02541] - Staining of paraffin-embedded Human pancreas tissue using anti-Glucose 6 phosphate isomerase mouse monoclonal antibody.
Glucose 6 phosphate isomerase Antibody (OTI2D2)

Glucose 6 phosphate isomerase Antibody (OTI2D2)[NBP2-02541]

Glucose 6 phosphate isomerase Antibody (OTI2D2)[NBP2-02541]Simple Western� analysis of endogenous protein ARNTL from HEK293T lysates (0.2 mg/mL) using ARNTL Mouse Monoclonal Antibody. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess� Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12�230 kDa Separation Module.

Applications for Glucose 6 phosphate isomerase Antibody (OTI2D2)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:1000-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.68 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Glucose 6 phosphate isomerase

This gene belongs to the GPI family whose members encode multifunctional phosphoglucose isomerase proteins involved in energy pathways. The protein encoded by this gene is a dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. The protein functions in different capacities inside and outside the cell. In the cytoplasm, the gene product is involved in glycolysis and gluconeogenesis, while outside the cell it functions as a neurotrophic factor for spinal and sensory neurons. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment.

Alternate Names

AMFGNPI, Autocrine motility factor, DKFZp686C13233, EC 5.3.1.9, glucose phosphate isomerase, glucose-6-phosphate isomerase, hexose monophosphate isomerase, hexosephosphate isomerase, Neuroleukin, NLKSA36, oxoisomerase, PGI, PHI, Phosphoglucose isomerase, phosphohexomutase, Phosphohexose isomerase, phosphosaccharomutase, SA-36, Sperm antigen 36, sperm antigen-36

Entrez Gene IDs

2821 (Human)

Gene Symbol

GPI

Additional Glucose 6 phosphate isomerase Products

Product Documents for Glucose 6 phosphate isomerase Antibody (OTI2D2)

Certificate of Analysis

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Product Specific Notices for Glucose 6 phosphate isomerase Antibody (OTI2D2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Glucose 6 phosphate isomerase Antibody (OTI2D2)

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Protocols

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