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A: The concentration of this protein (NBP1-72512) is 0.5mg/ml.

Recombinant Human Glutathione Peroxidase 1/GPX1 His Protein

Novus Biologicals | Catalog # NBP1-72512

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Applications

SDS-PAGE

View all Glutathione Peroxidase 1/GPX1 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-203 of Human Glutathione Peroxidase 1/GPX1

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MCAARLAAAA AAAQSVYAFS ARPLAGGEPV SLGSLRGKVL LIENVASLCG TTVRDYTQMN ELQRRLGPRG LVVLGFPCNQ FGHQENAKNE EILNSLKYVR PGGGFEPNFM LFEKCEVNGA GAHPLFAFLR EALPAPSDDA TALMTDPKLI TWSPVCRNDV AWNFEKFLVG PDGVPLRRYS RRFQTIDIEP DIEALLSQGP SCA

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

24.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Glutathione Peroxidase 1/GPX1 His Protein

SDS-PAGE: Recombinant Human Glutathione Peroxidase 1/GPX1 His Protein [NBP1-72512]

SDS-Page: Recombinant Human Glutathione Peroxidase 1/GPX1 Protein [NBP1-72512] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain. Molecular Weight: 24.2kDa (223aa), confirmed by MALDI-TOF

Formulation, Preparation, and Storage

NBP1-72512

Formulation	20 mM Tris-HCl buffer (pH 8.0), 2 mM DTT, 30% glycerol, 100 mM NaCl
Preservative	No Preservative
Concentration	0.5 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Glutathione Peroxidase 1/GPX1

GPX1 (Glutathione peroxidase 1) belongs to the glutathione peroxidase family, consisting of eight known glutathione peroxidases (Gpx1-8) in humans. Glutathione peroxidase functions in the detoxification of hydrogen peroxide, and is one of the most important antioxidant enzymes in humans. The GPX1 is part of the enzymatic antioxidant defence, preventing oxidative damage to DNA, proteins and lipids by detoxifying hydrogen and lipid peroxides that may contribute to prostate cancer development. This protein is one of only a few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by the nonsense (stop) codon TGA. Recombinant human GPX1(U49C) protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

CGPx, GPX1, GSHPX1

Gene Symbol

GPX1

Additional Glutathione Peroxidase 1/GPX1 Products

Product Documents for Recombinant Human Glutathione Peroxidase 1/GPX1 His Protein

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Certificate of Analysis

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Product Specific Notices for Recombinant Human Glutathione Peroxidase 1/GPX1 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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