Glypican 3 Antibody (CL12711) - BSA Free

Novus Biologicals | Catalog # NBP3-18542

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # CL12711

Format

BSA Free
View all Glypican 3 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a synthetic peptide derived from P51654, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Glypican 3 Antibody (CL12711) - BSA Free

Immunohistochemistry-Paraffin: Glypican 3 Antibody (CL12711) [NBP3-18542]

Immunohistochemistry-Paraffin: Glypican 3 Antibody (CL12711) [NBP3-18542]

Immunohistochemistry-Paraffin: Glypican 3 Antibody (CL12711) [NBP3-18542] - Analysis in human placenta and skeletal muscle tissues using NBP3-18542 antibody. Corresponding GPC3 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Glypican 3 Antibody (CL12711) [NBP3-18542]

Immunohistochemistry-Paraffin: Glypican 3 Antibody (CL12711) [NBP3-18542]

Immunohistochemistry-Paraffin: Glypican 3 Antibody (CL12711) [NBP3-18542] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: Glypican 3 Antibody (CL12711) [NBP3-18542]

Immunohistochemistry-Paraffin: Glypican 3 Antibody (CL12711) [NBP3-18542]

Immunohistochemistry-Paraffin: Glypican 3 Antibody (CL12711) [NBP3-18542] - Staining of human liver cancer (hepatocellular carcinoma) shows moderate membranous positivity in tumor cells.
Immunohistochemistry-Paraffin: Glypican 3 Antibody (CL12711) [NBP3-18542]

Immunohistochemistry-Paraffin: Glypican 3 Antibody (CL12711) [NBP3-18542]

Immunohistochemistry-Paraffin: Glypican 3 Antibody (CL12711) [NBP3-18542] - Staining of human ovarian cancer (papillary carcinoma) shows moderate membranous positivity in tumor cells.
Immunohistochemistry-Paraffin: Glypican 3 Antibody (CL12711) [NBP3-18542]

Immunohistochemistry-Paraffin: Glypican 3 Antibody (CL12711) [NBP3-18542]

Immunohistochemistry-Paraffin: Glypican 3 Antibody (CL12711) [NBP3-18542] - Staining of human testis cancer (seminoma) shows moderate membranous positivity in tumor cells.
Immunohistochemistry-Paraffin: Glypican 3 Antibody (CL12711) [NBP3-18542]

Immunohistochemistry-Paraffin: Glypican 3 Antibody (CL12711) [NBP3-18542]

Immunohistochemistry-Paraffin: Glypican 3 Antibody (CL12711) [NBP3-18542] - Staining of human ovarian cancer (yolk sack tumor) shows moderate membranous positivity in tumor cells.
Immunohistochemistry-Paraffin: Glypican 3 Antibody (CL12711) [NBP3-18542]

Immunohistochemistry-Paraffin: Glypican 3 Antibody (CL12711) [NBP3-18542]

Immunohistochemistry-Paraffin: Glypican 3 Antibody (CL12711) [NBP3-18542] - Staining of human placenta shows strong granular cytoplasmic positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: Glypican 3 Antibody (CL12711) [NBP3-18542]

Immunohistochemistry-Paraffin: Glypican 3 Antibody (CL12711) [NBP3-18542]

Immunohistochemistry-Paraffin: Glypican 3 Antibody (CL12711) [NBP3-18542] - Staining of human kidney shows moderate membranous positivity in cells in tubules.
Immunohistochemistry-Paraffin: Glypican 3 Antibody (CL12711) [NBP3-18542]

Immunohistochemistry-Paraffin: Glypican 3 Antibody (CL12711) [NBP3-18542]

Immunohistochemistry-Paraffin: Glypican 3 Antibody (CL12711) [NBP3-18542] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

Applications for Glypican 3 Antibody (CL12711) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 -1:2500
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Glypican 3

The Glypican 3 (GPC3) gene encodes for a member of the glypican-related integral membrane proteoglycan family (GRIPS). Two isoforms of the protein exist, isoform 1 at a length of 580 amino acids long and 65 kDA while isoform 2 is 526 amino acids long at 59 kDA. The protein encoded by GPC3 is thought to prevent the dipeptidyl peptidase activity of DPP4 and may participate in the suppression and regulation of growth in the predominately mesodermal tissues and organs. The GPC3 gene participates in the Wnt signaling pathway, metabolism, and visual phototransduction through interactions with genes FGF2, WNT3A, WNT7B, BCAN, and BGN. GPC3 has been researched in various diseases such as paine syndrome, horner's sundrome, liver cirrhosis, simpson-golabi-behmel syndrome, germ cell tumors, wilms tumors, complex regional pain syndrome, and hepatocellulcar carcinoma.

Alternate Names

GPC3

Gene Symbol

GPC3

Additional Glypican 3 Products

Product Documents for Glypican 3 Antibody (CL12711) - BSA Free

Certificate of Analysis

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Product Specific Notices for Glypican 3 Antibody (CL12711) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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