Key Product Details

Species Reactivity

Human

Applications

Western Blot, ICC/IF (Negative), Immunohistochemistry (Negative)

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 2059D

Format

Azide and BSA Free
View all GM130/GOLGA2 Primary Antibodies »

Product Specifications

Immunogen

Partial recombinant human GOLG-A2 protein (amino acids 528-606) [UniProt Q08379]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

>90% by SDS PAGE

Scientific Data Images for GM130/GOLGA2 Antibody (2059D) - Azide and BSA Free

Western Blot: GM130/GOLGA2 Antibody (2059D)Azide and BSA Free [MAB8199]

Western Blot: GM130/GOLGA2 Antibody (2059D)Azide and BSA Free [MAB8199]

Western Blot: GM130/GOLGA2 Antibody (2059D) [MAB8199] - Analysis of ZR-75 cell lysate with 0.5 ug/ml of hGM130 Monoclonal Antibody followed by HRP-conjugated Anti-Rabbit IgG Antibody. A specific band was detected for hGM130 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for GM130/GOLGA2 Antibody (2059D) - Azide and BSA Free

Application
Recommended Usage

ICC/IF (Negative)

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry (Negative)

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GM130/GOLGA2

Golgi Matrix Protein (GM130) is peripheral cytoplamic protein that is bound to the Golgi membranes. It has been implicated as a maintainer of cis-Golgi structure (1). During mitosis, it regulates the disassembly and reassembly of the Golgi complex. It is also linked to docking and fusion of coatomer (COP I) coated vesicles to the Golgi membrane (2). The COOH-terminal domain of GM130 is highly homologous to Golgi human auto-antigen, golgin-95. GM130 interacts specifically and GTP-dependent manner with Rab1b protein, a regulator of anterograde traffic between ER and Golgi membranes (3). GM130 is also activator of YSK1, an essential player in cell migration.

Long Name

Golgin A2

Alternate Names

GOLGA2, Golgin-95, SY11 Protein

Gene Symbol

GOLGA2

Additional GM130/GOLGA2 Products

Product Documents for GM130/GOLGA2 Antibody (2059D) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for GM130/GOLGA2 Antibody (2059D) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Inflammatory Mediators

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